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. 2022 Jul 14:2022:6209047.
doi: 10.1155/2022/6209047. eCollection 2022.

Screening of Proliferation-Related Genes and Pathological Changes in Thiram-Induced Tibial Dyschondroplasia

Ali Raza Jahejo  1   2 , Chen-Liang Zhang  1 , Fazul Nabi  3 , Nazeer Hussain Kalhoro  2 , Qurban Ali Shah  3 , Zohaib Ahmed Bhutto  3 , Jin-Feng Zhao  1 , Jin Yu  1 , Guan-Bao Ning  1 , Ding Zhang  1 , Shu-Ming Chen  1 , Wen-Xia Tian  1
Affiliations

Screening of Proliferation-Related Genes and Pathological Changes in Thiram-Induced Tibial Dyschondroplasia

Ali Raza Jahejo et al. Biomed Res Int. .

Abstract

Materials and methods: Three hundred sixty (n = 360) broiler chickens were equally divided into control (C) and thiram (T) groups. Furthermore, the C and T groups were dividedinto 8-, 9-, 11-, and 13-day-old chickens.

Results: Clinically, it was observed that broiler chickens of group T had abnormal posture, gait, and lameness, and histopathological results revealed dead and abnormal chondrocytes of T group on day 6. Real-time qPCR results showed that HDAC1, MTA1, H4, and PCNA genes were significantly expressed (P < 0.05). HDAC1 was upregulated on days 1, 2, 4, and 6 (P < 0.01); MTA1 was upregulated on days 1 and 2 (P < 0.01); H4 was upregulated on days 2 and 4 (P < 0.01), and PCNA was downregulated on days 1, 2, and 4 (P < 0.01). Furthermore, IHC results of HDAC1 protein were significantly (P < 0.01) expressed in proliferative zone of day 1 and hypertrophic zone of day 6. MTA1 protein was significantly (P < 0.01) expressed on days 1, 2, and 6 in all zones, except prehypertrophic zone of day 2.

Conclusion: In conclusion, the mRNA expressions of HDAC1, MTA1, H4, and PCNA were differentially expressed in the chondrocytes of thiram-induced TD chickens. HDAC1 and MTA1 protein expression found involved and responsible in the abnormal chondrocytes' proliferation of broiler chicken.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
Clinical observation of lameness in control vs. thiram- induced tibial dyschondroplasia (TD) in chicks on days 1, 2, 4, and 6.
Figure 2
Figure 2
Comparison of average TD lesion score in control vs. thiram-induced TD in chicks on days 1, 2, 4, and 6. C = control; T = thiram. Following Pines et al. [31], the average TD score was calculated.
Figure 3
Figure 3
Comparison of the growth plate in control vs. and thiram-induced TD broiler chickens on days 1, 2, 4, and 6. C = control; T = thiram; GP = growth plate; BV = blood vessels; TDL = tibial dyschondroplasia lesions; AC = articular cartilage.
Figure 4
Figure 4
The histological characteristics of chondrocytes in the different zones of the tibia growth plate on days 1, 2, 4, and 6 in control vs. thiram-induced TD. C = control; T = thiram; A = articular zone; P = proliferative zone; PH = prehypertrophic zone; H = hypertrophic zone (bar = 50 μm).
Figure 5
Figure 5
Analysis of gene expression. (a). Analysis of RNA detected by agarose gel electrophoresis. (b). Gene expression analysis by qRT-PCR. C = control; C1 = control day 1; T1 = thiram day 1; C2 = control day 2; T2 = thiram day 2; C4 = control day 4; T4 = thiram day 4; C4 = control day 4; T4 = thiram day 4. ∗ indicates significant differences (P < 0.05).
Figure 6
Figure 6
The immunohistochemistry analysis of HDAC1 protein expression in the tibial growth plate of different chondrocytes zones on days 1, 2, 4, and 6 in control and thiram-induced tibial dyschondroplasia. C = control; T = thiram; A = articular zone; P = proliferative zone; PH = prehypertrophic zone; H = hypertrophic zone (bar = 50 μm).
Figure 7
Figure 7
The immunohistochemistry analysis of MTA1 protein expression in the tibial growth plate of different chondrocytes zones on days 1, 2, 4, and 6 in control and thiram-induced tibial dyschondroplasia. C = control; T = thiram; A = articular zone; P = proliferative zone; PH = prehypertrophic zone; H = hypertrophic zone (bar = 400 μm).
See this image and copyright information in PMC

References

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