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. 2021 Dec;140(6):1081-1089.
doi: 10.1111/pbr.12969. Epub 2021 Oct 9.

Introgression of bruchid (Zabrotes subfasciatus) resistance into small red common bean (Phaseolus vulgaris) background and validation of the BRU_00261 (snpPV0007) resistance marker

Shiferaw Girsil Tigist  1 , Bodo Raatz  2 , Amelework Assefa  3 , Rob Melis  4 , Julia Sibiya  4 , Gemechu Keneni  5 , Clare Mukankusi  6 , Berhanu Fenta  1 , Selamawit Ketema  1 , Dagmawit Tsegaye  1
Affiliations

Introgression of bruchid (Zabrotes subfasciatus) resistance into small red common bean (Phaseolus vulgaris) background and validation of the BRU_00261 (snpPV0007) resistance marker

Shiferaw Girsil Tigist et al. Plant Breed. 2021 Dec.

Abstract

Bruchids are a major storage pest of common bean. Genetic resistance is a suitable method to avoid grain losses during storage. The objective of the study was to introgress the arcelin-based resistance locus into selected advanced breeding line and to validate the molecular marker BRU_00261. A total of 208 progeny F4 families were phenotyped using a randomized complete block design, with three replications. Highly significant differences (P < .001) among the entries, parents and offspring were recorded for almost all traits. There was no significant difference between the two parents in the number of eggs laid. The progenies were grouped as highly resistant (34.3%), resistant (11.9%), moderately resistant (21.4%) and susceptible (32.4%). The levels of broad sense heritability ranged from 68.5%-93.9% for all the traits. Eighty-three most resistant lines and the parental lines were genotyped with the marker BRU_00261 (snpPV0007). The marker segregation deviated significantly from the expected independent segregation towards a strong enrichment for the resistant marker in the selected families. This marker will be useful for selecting promising materials in early generations and phenotypic confirmation of positive lines in later generations.

Keywords: Mexican bean weevil; arcelin gene; bruchids; common bean; molecular marker.

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Conflict of interest statement

The authors have no any conflict of interest to declare.

Figures

FIGURE 1
FIGURE 1
Classification of progeny genotypes, based on bruchid susceptibility classes using percentage adult emergence: HR, highly resistant; R, resistant; RI, intermediate resistant; S, susceptible F4 families [Color figure can be viewed at wileyonlinelibrary.com]
FIGURE 2
FIGURE 2
Boxplots of five bruchid response traits evaluated in 208 lines grouped by level of resistance. Level of resistance abbreviations are as follows: HR, highly resistance; R, resistant; RI, intermediate resistant; S, susceptible [Color figure can be viewed at wileyonlinelibrary.com]
FIGURE 3
FIGURE 3
Phenotypic correlations among different response characters to bean bruchids infestation in 85 common bean lines: NE, number of egg; NAE, number of adults emerged; PAE, percentage adult emergence; PD, percent seed damage; SWL, seed weight loss [Color figure can be viewed at wileyonlinelibrary.com]
FIGURE 4
FIGURE 4
Expected and observed segregation for the bruchid resistance marker BRU_00261 tagging the arcelin locus in the 83 F4 lines of the SCR15 × MAZ200 population selected for bruchid resistance [Color figure can be viewed at wileyonlinelibrary.com]
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