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. 2023 Apr;41(4):815-822.
doi: 10.1002/jor.25422. Epub 2022 Aug 6.

The effect of surgical revascularization on the mechanical properties of cryopreserved bone allograft in a porcine tibia model

Noortje Visser  1   2 , Elisa Rezaie  1   2 , Alexandra Ducharme  1 , Alexander Y Shin  1 , Allen T Bishop  1
Affiliations
Free article

The effect of surgical revascularization on the mechanical properties of cryopreserved bone allograft in a porcine tibia model

Noortje Visser et al. J Orthop Res. 2023 Apr.
Free article

Abstract

Cryopreserved bone allografts(CBA) are susceptible to infection, nonunion, and late stress fracture. Although surgical revascularization by intramedullary implantation of an arteriovenous bundle (AV bundle) generates a neoangiogenic blood supply, there is potential for vascular ingrowth-mediated bone resorption to weaken the graft. For this reason, we have evaluated changes in CBA mechanical properties of structural tibial allografts with and without surgically induced angiogenesis. Cryopreserved tibia bone allografts were transplanted to reconstruct a 3.5 cm segmental tibial defect in 16 Yucatan mini pigs. Surgical revascularization was performed in half by implantation of a cranial tibial AV bundle, (revascularization group). A control group of identical size had a ligated AV bundle implanted, (ligated group). At 20 weeks micro-computed tomography (CT) measured bone mineral density (BMD) as well as bone union. Reference point indentation (RPI) compared cortex material properties, and axial compression determined the allotransplant compressive modulus. Seven of eight tibiae in the angiogenesis group were healed at both junction points at 20 weeks. Only four of eight tibiae healed in the ligated control group. There was no significant difference between the revascularization and ligated control groups in BMD and axial compression test. Similarly, RPI parameters were statistically equal. In paired comparisons with contralateral tibias, however, some RPI values were significantly worse in the ligated control group tibiae. This study demonstrates no adverse effect of surgical angiogenesis on cryopreserved structural bone allograft biomechanical properties in a large animal orthotopic segmental tibial defect model. These data suggest the potential value of surgical angiogenesis in clinical limb-sparing reconstructive surgery.

Keywords: allograft; angiogenesis; biomechanics; bone; reference point indentation.

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References

REFERENCES

    1. de Boer HH, Wood MB. Bone changes in the vascularised fibular graft. J Bone Joint Surg Br. 1989;71:374-378.
    1. Delloye C, Cornu O, Druez V, Barbier O. Bone allografts: what they can offer and what they cannot. J Bone Joint Surg Br. 2007;89:574-579.
    1. Dick HM, Strauch RJ. Infection of massive bone allografts. Clin Orthop Relat Res. 1994;306:46-53.
    1. Enneking WF, Eady JL, Burchardt H. Autogenous cortical bone grafts in the reconstruction of segmental skeletal defects. J Bone Joint Surg Am. 1980;62:1039-1058.
    1. Fox EJ, Hau MA, Gebhardt MC, Hornicek FJ, Tomford WW, Mankin HJ. Long-term followup of proximal femoral allografts. Clin Orthop Relat Res. 2002;397:106-113.

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