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. 2022 Sep;87(9):e202200133.
doi: 10.1002/cplu.202200133. Epub 2022 Jul 26.

Amphiphilic Cationic Phthalocyanines for Photodynamic Therapy of Cancer

Marie Halaskova  1 , Filip Kostelansky  2 , Jiri Demuth  2 , Ingrid Hlbocanova  1 , Miroslav Miletin  2 , Petr Zimcik  2 , Miloslav Machacek  1 , Veronika Novakova  2
Affiliations

Amphiphilic Cationic Phthalocyanines for Photodynamic Therapy of Cancer

Marie Halaskova et al. Chempluschem. 2022 Sep.

Abstract

Effective interaction with biomembranes is essential for activity of photosensitizers; however, majority of them are highly charged symmetrical species. Amphiphilic cationic phthalocyanines differing in bulkiness of substitution on lipophilic part (-H, -SMe, -StBu) were therefore prepared. Compounds had high singlet oxygen production (ΦΔ =0.38-0.46, DMSO), good fluorescence emission (ΦF =0.21-0.26, DMSO), and log P values ranging -0.07-1.1 (1-octanol/PBS). Study of interaction with liposomes revealed that also bulky -StBu derivatives are able to enter biomembranes. Detail in vitro studies (toxicity, subcellular localization, type of cell death, and morphology) were performed. Compounds were characterized by excellent EC50 values in range of dozens of nM (HeLa, EA.hy926, MCF-7, HCT116), which were dependent on drug-light interval and reached plateau after 4 h on HeLa cells. Well-balanced lipophilicity with ability to interact with biomembranes rank these derivatives among perspective photosensitizers, even for vascular-targeted PDT (VTP) since they kill EA.hy926 without any preincubation time.

Keywords: fluorescence; liposomes; photodynamic therapy; phthalocyanine; singlet oxygen.

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References

    1. None
    1. C. G. Claessens, U. Hahn, T. Torres, Chem. Rec. 2008, 8, 75–97;
    1. R. C. H. Wong, P. C. Lo, D. K. P. Ng, Coord. Chem. Rev. 2019, 379, 30–46;
    1. M. Urbani, M. E. Ragoussi, M. K. Nazeeruddin, T. Torres, Coord. Chem. Rev. 2019, 381, 1–64;
    1. G. Simonneaux, P. Tagliatesta, J. Porphyrins Phthalocyanines 2004, 8, 1166–1171;

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