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. 2022:2470:689-713.
doi: 10.1007/978-1-0716-2189-9_52.

Assessment of Plasmodium falciparum Antigen-Specific B Cells

Christine Sarah Hopp  1 , Justin James Taylor  2 , Peter Dobbs Crompton  3
Affiliations

Assessment of Plasmodium falciparum Antigen-Specific B Cells

Christine Sarah Hopp et al. Methods Mol Biol. 2022.

Abstract

This protocol describes methods that exploit the specificity of binding between the B cell receptor and cognate antigen to detect and characterize Plasmodium-specific human B cells. Importantly, this approach allows for the isolation and study of B cells without activating the cells or requiring them to secrete antibodies. The protocol describes how antigen "probes" are generated and used in flow cytometry to identify and sort antigen-specific B cells, and includes methods for enrichment of antigen-specific B cells prior to flow cytometry. Finally, we detail techniques to optimize the exclusion of B cells that are not specific for the antigen of interest.

Keywords: Antibody; Antigen-specific; Antigens; B cell receptor; B cells; Flow cytometry; Humoral immune response; Malaria; Probe; Tetramer; Vaccine.

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