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. 2022 Jun 24;11(7):845.
doi: 10.3390/antibiotics11070845.

A Universal LC-MS/MS Method for Simultaneous Detection of Antibiotic Residues in Animal and Environmental Samples

Chak-Lun Chan  1   2 , Hogan Kok-Fung Wai  1   2 , Peng Wu  1 , Siu-Wai Lai  1 , Olivia Sinn-Kay Chan  1 , Hein M Tun  1   2   3   4
Affiliations

A Universal LC-MS/MS Method for Simultaneous Detection of Antibiotic Residues in Animal and Environmental Samples

Chak-Lun Chan et al. Antibiotics (Basel). .

Abstract

Detecting and monitoring the usage of antibiotics is a critical aspect of efforts to combat antimicrobial resistance. Antibiotic residue testing with existing LC-MS/MS methods is limited in detection range. Current methods also lack the capacity to detect multiple antibiotic residues in different samples simultaneously. In this study, we demonstrate a methodology that permits simultaneous extraction and detection of antibiotic residues in animal and environmental samples. A total of 30 different antibiotics from 13 classes could be qualitatively detected with our methodology. Further study to reduce analytes' matrix effect would allow for quantification of antibiotic residues.

Keywords: LC-MS/MS; antibiotic residue; solid-phase extraction.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Flowchart showing the key steps in antibiotic residue testing. The figure was created with BioRender.com.
See this image and copyright information in PMC

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