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. 2022 Jul 13;14(14):3397.
doi: 10.3390/cancers14143397.

Unraveling the Risk Factors and Etiology of the Canine Oral Mucosal Melanoma: Results of an Epidemiological Questionnaire, Oral Microbiome Analysis and Investigation of Papillomavirus Infection

Joyce Pires de Carvalho  1 , Marcella Collaneri Carrilho  1 , Denner Santos Dos Anjos  2 , Carolina Dagli Hernandez  3 , Laura Sichero  4 , Maria Lúcia Zaidan Dagli  1
Affiliations

Unraveling the Risk Factors and Etiology of the Canine Oral Mucosal Melanoma: Results of an Epidemiological Questionnaire, Oral Microbiome Analysis and Investigation of Papillomavirus Infection

Joyce Pires de Carvalho et al. Cancers (Basel). .

Abstract

Oral mucosal melanoma (OMM) is the most common oral cancer in dogs and is very aggressive in this species; its risk factors and etiology are yet to be determined. This study aimed to unravel the risk factors for the development of OMM in dogs and to investigate the possible presence of papillomaviruses as an etiological factor. A case-control study was conducted in 15 dogs with OMM and 15 paired controls whose owners answered an epidemiological questionnaire. Oral swabs from the same dogs were subjected to 16S rRNA sequencing for microbiome analyses. In addition, DNA fragments of OMM had their DNA extracted and amplified by polymerase chain reaction in an attempt to detect canine papillomaviruses. The gingiva was the most frequent anatomical site (47%) of OMM, and most tumors were stage III when diagnosed. Most dogs bearing OMM and the controls had grade 3 periodontal disease, and this factor, along with tartar treatment and tooth brushing, did not differ between cases and controls. Most dogs with OMM and most controls had contact with smokers; there was no statistically significant difference. Canine papillomaviruses were not detected among OMM cases. Tannerella forsythia and Porphyromonas gingivalis were significantly increased in case dogs compared to the controls. As these bacteria are reportedly involved in the pathogenesis of periodontal disease and esophageal cancer in humans, we suggest that they might be risk factors for the development of canine OMM. The limitations of this study include the low number of dogs, and therefore, further studies on canine OMM with larger numbers of animals are encouraged.

Keywords: dogs; melanoma; microbiome.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Bacterial phyla found in the oral cavities of dogs bearing OMM. * indicates that the values are significantly different.
Figure 2
Figure 2
Bacterial species found in the oral cavities of dogs bearing OMM.
See this image and copyright information in PMC

References

    1. Feller L., Khammissa R.A.G., Lemmer J. A Review of the Aetiopathogenesis and Clinical and Histopathological Features of Oral Mucosal Melanoma. Sci. World J. 2017;2017:9189812. doi: 10.1155/2017/9189812. - DOI - PMC - PubMed
    1. Todoroff R.J., Brodey R.S. Oral and Pharyngeal Neoplasia in the Dog: A Retrospective Survey of 361 Cases. J. Am. Vet. Med. Assoc. 1979;175:567–571. - PubMed
    1. Ramos-Vara J.A., Beissenherz M.E., Miller M.A., Johnson G.C., Pace L.W., Fard A., Kottler S.J. Retrospective Study of 338 Canine Oral Melanomas with Clinical, Histologic, and Immunohistochemical Review of 129 Cases. Vet. Pathol. 2000;37:597–608. doi: 10.1354/vp.37-6-597. - DOI - PubMed
    1. Dobson J.M., Samuel S., Milstein H., Rogers K., Wood J.L. Canine Neoplasia in the UK: Estimates of Incidence Rates from a Population of Insured Dogs. J. Small Anim. Pract. 2002;43:240–246. doi: 10.1111/j.1748-5827.2002.tb00066.x. - DOI - PubMed
    1. Smith S.H., Goldschmidt M.H., McManus P.M. A Comparative Review of Melanocytic Neoplasms. Vet. Pathol. 2002;39:651–678. doi: 10.1354/vp.39-6-651. - DOI - PubMed