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Guideline
. 2022 Jul 12;12(7):1698.
doi: 10.3390/diagnostics12071698.

Perls' Stain Guidelines from the French-Speaking Cellular Hematology Group (GFHC)

Camille Lours  1 , Laurane Cottin  2 , Margaux Wiber  2 , Valérie Andrieu  3 , Véronique Baccini  4 , Lucile Baseggio  5 , Chantal Brouzes  6 , Bernard Chatelain  7 , Sylvie Daliphard  8 , Odile Fenneteau  9 , Franck Geneviève  2 , Sandrine Girard  1 , Vincent Leymarie  10 , Karim Maloum  11 , Jean-Baptiste Rieu  12 , Gérard Sebahoun  13 , Isabelle Sudaka  14 , Xavier Troussard  15 , Orianne Wagner-Ballon  16 , Soraya Wuilleme  17 , Valérie Bardet  18 , Jean-François Lesesve  19
Affiliations
Guideline

Perls' Stain Guidelines from the French-Speaking Cellular Hematology Group (GFHC)

Camille Lours et al. Diagnostics (Basel). .

Abstract

In order to standardize cellular hematology practices, the French-speaking Cellular Hematology Group (Groupe Francophone d'Hématologie Cellulaire, GFHC) focused on Perls' stain. A national survey was carried out, leading to the proposal of recommendations on insoluble iron detection and quantification in bone marrow. The criteria presented here met with a "strong professional agreement" and follow the suggestions of the World Health Organization's classification of hematological malignancies.

Keywords: Perls’ stain; Prussian blue stain; bone marrow; cytochemistry; iron staining; iron store; myelodysplastic syndromes; recommendations; sideroblasts.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The different types of sideroblasts: (A): Three type 0 sideroblasts, (B,C): One type 1 and one Ring Sideroblast (or type 3), (D): One type 1 sideroblast, (E,F): One type 2 sideroblast, (G,H): One Ring Sideroblast.
Figure 2
Figure 2
Prussian blue reaction of erythroid precursors, hematoxylin counterstain. (A,B) medullar iron content evaluation; (C,D) extracellular iron deposits; (E–G) macrophages; (H) siderocyte; (I) sideroblast (a) and siderocyte (b); and (J) hemosiderin-containing plasma cell (a) and one siderocyte (b).
Figure 3
Figure 3
Technical protocol of Perls’ Stain.
See this image and copyright information in PMC

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