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Randomized Controlled Trial
. 2022 Jul 17;23(14):7888.
doi: 10.3390/ijms23147888.

Varying Oxygen Partial Pressure Elicits Blood-Borne Microparticles Expressing Different Cell-Specific Proteins-Toward a Targeted Use of Oxygen?

Costantino Balestra  1   2   3   4 , Awadhesh K Arya  5 , Clément Leveque  1 , Fabio Virgili  6 , Peter Germonpré  1   3   7 , Kate Lambrechts  1 , Pierre Lafère  1   3 , Stephen R Thom  5
Affiliations
Randomized Controlled Trial

Varying Oxygen Partial Pressure Elicits Blood-Borne Microparticles Expressing Different Cell-Specific Proteins-Toward a Targeted Use of Oxygen?

Costantino Balestra et al. Int J Mol Sci. .

Abstract

Oxygen is a powerful trigger for cellular reactions, but there are few comparative investigations assessing the effects over a large range of partial pressures. We investigated a metabolic response to single exposures to either normobaric (10%, 15%, 30%, 100%) or hyperbaric (1.4 ATA, 2.5 ATA) oxygen. Forty-eight healthy subjects (32 males/16 females; age: 43.7 ± 13.4 years, height: 172.7 ± 10.07 cm; weight 68.4 ± 15.7 kg) were randomly assigned, and blood samples were taken before and 2 h after each exposure. Microparticles (MPs) expressing proteins specific to different cells were analyzed, including platelets (CD41), neutrophils (CD66b), endothelial cells (CD146), and microglia (TMEM). Phalloidin binding and thrombospondin-1 (TSP), which are related to neutrophil and platelet activation, respectively, were also analyzed. The responses were found to be different and sometimes opposite. Significant elevations were identified for MPs expressing CD41, CD66b, TMEM, and phalloidin binding in all conditions but for 1.4 ATA, which elicited significant decreases. Few changes were found for CD146 and TSP. Regarding OPB, further investigation is needed to fully understand the future applications of such findings.

Keywords: altitude; cellular reactions; decompression sickness; diving; hyperbaric oxygen; hyperoxia; hyperoxic-hypoxic paradox; hypoxia; normobaric oxygen paradox.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Total microparticle response following different oxygen levels. Box and Whisker plots indicating median, 1st quartile, 3rd quartile, interquartile range, min., and max. in comparison to baseline (before oxygen exposure), which was set at 100%. (One-sample t test: **** p < 0.0001, *** p < 0.001, * p < 0.05, ns = non-significant).
Figure 2
Figure 2
CD41+ response following different oxygen levels exposure. Box and Whisker plots indicating median, 1st quartile, 3rd quartile, interquartile range, min., and max. in comparison to baseline (before oxygen exposure), which was set at 100%. (One-sample t test: ** p < 0.01, * p < 0.05, ns = non-significant).
Figure 3
Figure 3
CD66b+ response following different oxygen levels exposure. Box and Whisker plots indicating median, 1st quartile, 3rd quartile, interquartile range, min., and max. in comparison to baseline (before oxygen exposure), which was set at 100%. (One-sample t test: **** p < 0.0001, *** p < 0.001, ** p < 0.01, ns = non-significant).
Figure 4
Figure 4
CD146+ response following different oxygen levels exposure. Box and Whisker plots indicating median, 1st quartile, 3rd quartile, interquartile range, min., and max. in comparison to baseline (before oxygen exposure), which was set at 100%. (One-sample t test: ** p < 0.01, * p < 0.05, ns = non-significant).
Figure 5
Figure 5
TMEM119+ response following different oxygen levels exposure. Box and Whisker plots indicating median, 1st quartile, 3rd quartile, interquartile range, min., and max. in comparison to baseline (before oxygen exposure), which was set at 100%. (One-sample t test: ** p < 0.01, * p < 0.05, ns = non-significant).
Figure 6
Figure 6
Thrombospondin-1 response following different oxygen levels exposure. Box and Whisker plots indicating median, 1st quartile, 3rd quartile, interquartile range, min., and max. in comparison to baseline (before oxygen exposure), which was set at 100%. (One-sample t test: * p < 0.05, ns = non-significant).
Figure 7
Figure 7
Phalloidin response following different oxygen levels exposure. Box and Whisker plots indicating median, 1st quartile, 3rd quartile, interquartile range, min., and max. in comparison to baseline (before oxygen exposure), which was set at 100%. (One-sample t test: *** p < 0.001, * p < 0.05, ns = non-significant).
Figure 8
Figure 8
Percentual variations in MPs after 60 min of oxygen breathing. Levels of oxygen are shown on the ordinate, and Total MPs and MP sub-types are shown on the abscissa. Blood sampling occurred 120 min after exposures (in total 48 subjects participated to the experiment). Results are expressed in the heat map as mean percentage change.
Figure 9
Figure 9
Experimental flowchart.
See this image and copyright information in PMC

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