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. 2022 Jun 22;10(7):1272.
doi: 10.3390/microorganisms10071272.

Production and Characterization of Rhamnolipids Produced by Pseudomonas aeruginosa DBM 3774: Response Surface Methodology Approach

Olga Maťátková  1 , Jana Michailidu  1 , Richard Ježdík  1 , Irena Jarošová Kolouchová  1 , Tomáš Řezanka  2 , Vladimír Jirků  1 , Jan Masák  1
Affiliations

Production and Characterization of Rhamnolipids Produced by Pseudomonas aeruginosa DBM 3774: Response Surface Methodology Approach

Olga Maťátková et al. Microorganisms. .

Abstract

Rhamnolipids are extensively studied biosurfactants due to their potential in many industrial applications, eco-friendly production and properties. However, their availability for broader application is severely limited by their production costs, therefore the optimization of efficacy of their cultivation gains significance as well as the information regarding the physio-chemical properties of rhamnolipids resulting from various cultivation strategies. In this work, the bioprocess design focused on optimization of the rhamnolipid yield of Pseudomonas aeruginosa DBM 3774 utilizing the response surface methodology (RSM). Six carbon sources were investigated for their effect on the rhamnolipid production. The RSM prediction improved the total rhamnolipid yield from 2.2 to 13.5 g/L and the rhamnolipid productivity from 11.6 to 45.3 mg/L/h. A significant effect of the carbon source type, concentration and the C/N ratio on the composition of the rhamnolipid congeners has been demonstrated for cultivation of P. aeruginosa DBM 3774 in batch cultivation. Especially, changes in presence of saturated fatty acid in the rhamnolipid congeners, ranging from 18.8% of unsaturated fatty acids (carbon source glycerol; 40 g/L) to 0% (sodium citrate 20 g/L) were observed. This demonstrates possibilities of model based systems as basis in cultivation of industrially important compounds like biosurfactants rhamnolipids and the importance of detailed study of interconnection between cultivation conditions and rhamnolipid mixture composition and properties.

Keywords: Pseudomonas aeruginosa; biosurfactants; fractional factorial design.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
3D surface plots obtained from the regression models based on the RSM designed experiments, showing the effect of glycerol (X1) and NaNO3 (X2) on the rhamnolipid yield (A) and rhamnolipid productivity (B).
Figure 2
Figure 2
Emulsification activity of the rhamnolipid mixtures obtained from cultivation of Pseudomonas aeruginosa DBM 3774 in the RSM predicted and the control medium: (■) hexane, (■) crude oil, (■) sunflower oil. Error bars represent standard deviation.
Figure 3
Figure 3
Phenanthrene solubilization with rhamnolipid mixtures produced by Pseudomonas aeruginosa DBM 3774 on different medium composition. (■) rhamnolipid from the RSM predicted medium, (×) rhamnolipid from the control medium.
See this image and copyright information in PMC

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