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. 2022 Jul 15;14(14):2873.
doi: 10.3390/polym14142873.

Effects of Drug-Free Pectin Hydrogel Films on Thermal Burn Wounds in Streptozotocin-Induced Diabetic Rats

Nur Nadhirah Nordin  1 , Nur Karimah Aziz  1 , Idanawati Naharudin  1   2 , Nor Khaizan Anuar  1   2   3
Affiliations

Effects of Drug-Free Pectin Hydrogel Films on Thermal Burn Wounds in Streptozotocin-Induced Diabetic Rats

Nur Nadhirah Nordin et al. Polymers (Basel). .

Abstract

This study aims to examine the influence of drug-free pectin hydrogel films on partial-thickness burn wounds using streptozotocin-induced diabetic rats as the animal model. Thirty male Sprague Dawley rats were included in the wound healing study, and scalding water was used to produce wounds in the dorsum region of the rats. Two different formulations of pectin hydrogel films, PH 2.5% and PH 5%, were prepared using a solvent evaporation method. MEBO® (moist exposed burn ointment), a commercial herbal formulation was used as a positive control. The progress of the wound healing was observed and compared between untreated normal rats, untreated diabetic rats, diabetic rats treated with MEBO®, diabetic rats treated with PH 2.5%, and diabetic rats treated with PH 5%. The results showed that diabetic rats treated with PH 5% healed faster than the untreated diabetic rats and diabetic rats treated with PH 2.5%. Interestingly, the diabetic rats treated with PH 5% healed as well as diabetic rats treated with MEBO®, where wounds were healed entirely on day 20. Nevertheless, both PH 2.5% and PH 5% showed a greater zone of inhibition than MEBO® when tested against Staphylococcus aureus.

Keywords: diabetic rats; drug-free; hydrogel; pectin; wound healing.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Chemical structure of (a) pectin and (b) pectin hydrogel film.
Figure 2
Figure 2
DSC thermograms of (a) unprocessed pectin and (b) pectin hydrogel film.
Figure 3
Figure 3
FTIR spectra of (a) unprocessed pectin and (b) pectin hydrogel film.
Figure 4
Figure 4
Photographs of wounds for various animal groups: untreated normal rats, untreated diabetic rats, and diabetic rats treated with MEBO®, PH 2.5%, and PH 5%.
Figure 5
Figure 5
Profiles of wound size.
Figure 6
Figure 6
Rate of re-epithelialisation.
Figure 7
Figure 7
Photomicrographs of the H&E-stained histology section for (a) diabetic rats at day 0, (b) untreated normal rats at day 20, (c) untreated diabetic rats at day 20, (d) diabetic rats treated with MEBO® at day 20, (e) diabetic rats treated with PH 2.5% at day 20, and (f) diabetic rats treated with PH 5% at day 20. Scale bar = 500 µm.
Figure 8
Figure 8
Antimicrobial activity of (a) negative control, (b) MEBO®, (c) PH 2.5%, and (d) PH 5% against S. aureus represented by the zone of inhibition.
See this image and copyright information in PMC

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