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. 2022 Aug 31;10(4):e0234622.
doi: 10.1128/spectrum.02346-22. Epub 2022 Jul 27.

Mycobacterium abscessus Tetracycline-Modifying Monooxygenase MAB_1496c Appears Not to Be Sufficient to Cause Resistance to Tetracycline When Expressed in Mycobacterium smegmatis

Noga Naor  1 , Erez Zarbib  2 , Daniel Barkan  1
Affiliations

Mycobacterium abscessus Tetracycline-Modifying Monooxygenase MAB_1496c Appears Not to Be Sufficient to Cause Resistance to Tetracycline When Expressed in Mycobacterium smegmatis

Noga Naor et al. Microbiol Spectr. .
No abstract available

Keywords: Mycobacterium; Mycobacterium abscessus; antibiotic resistance; drug resistance mechanisms; mycobacteria; tetracycline.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

FIG 1
FIG 1
(A) Genetic sequences of the MOP promoter (green) and untranslated region (UTR) (yellow) driving the expression of an HA-tagged (blue) TetX protein (red). (B) Western blot demonstrating the full-length (53.2-kDa) HA-TetX protein in mDB335 in comparison to WT bacteria. (Ci) Approximately 250 CFU of M. smegmatisempty kana vector (top) or mDB335 (bottom) was plated onto 7H10 agar plates with increasing concentrations of tetracycline. (Cii) WT M. smegmatis and M. smegmatis mDB335 were grown in 7H9/glycerol broth with the designated concentrations of tetracycline. (D) M. abscessus ATCC 19977 was plated onto 7H10 agar plates with the designated concentrations of tetracycline.
See this image and copyright information in PMC

References

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