Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Jul 23;27(15):4707.
doi: 10.3390/molecules27154707.

Biological Evaluation, Phytochemical Screening, and Fabrication of Indigofera Linifolia Leaves Extract-Loaded Nanoparticles

Muhammad Talha  1 , Noor Ul Islam  2 , Muhammad Zahoor  1 , Abdul Sadiq  3 , Asif Nawaz  3 , Farhat Ali Khan  4 , Naila Gulfam  5 , Saleh A Alshamrani  6 , Mohammed H Nahari  7 , Mohammed Abdulrahman Alshahrani  8 , Mater H Mahnashi  9 , Syed Shams Ul Hassan  10   11
Affiliations

Biological Evaluation, Phytochemical Screening, and Fabrication of Indigofera Linifolia Leaves Extract-Loaded Nanoparticles

Muhammad Talha et al. Molecules. .

Abstract

Indigofera linifolia is a medicinally important plant, and by virtue of its rich phytochemical composition, this plant is widely used as essential component in traditional medication systems. Due to its wide range of medicinal applications, the extract-loaded chitosan (Ext+Ch), extract-loaded PEG (Ext+PEG), and extract-loaded locust bean gum (Ext+LGB) nanoparticles (NPs) were prepared in the present study. The prepared NPs were then evaluated for their antibacterial, antioxidant, and antidiabetic potentials. Antibacterial activities of the crude extract and the synthesized NPs were performed following standard procedures reported in the literature. The antioxidant capabilities of extract and NPs were evaluated using DPPH free radical scavenging assay. The antidiabetic potential of the samples was evaluated against α-amylase and α-glucosidase. Ext+PEG NPs showed more potent antibacterial activity against the selected strains of bacteria with the highest activity against Escherichia coli. The lowest antibacterial potential was observed for Ext+LGB NPs. The Ext+LGB NPs IC50 value of 39 μg/mL was found to be the most potent inhibitor of DPPH free radicals. Ext+LGB NPs showed a greater extent of inhibition against α-glucosidase and α-amylase with an IC50 of 83 and 78 μg/mL, whereas for the standard acarbose the IC50 values recorded against the mentioned enzymes were 69 and 74 μg/mL, respectively. A high concentration of phenolics and flavonoids in the crude extract was confirmed through TPC and TFC tests, HPLC profiling, and GC-MS analysis. It was considered that the observed antibacterial, antidiabetic, and antioxidant potential might be due the presence of these phenolics and flavonoids detected. The plant could thus be considered as a potential candidate to be used as a remedy of the mentioned health complications. However, further research in this regard is needed to isolate the exact responsible compounds of the observed biological potentials exhibited by the crude extract. Further, toxicity and pharmacological evaluations in animal models are also needed to establish the safety or toxicity profile of the plant.

Keywords: Indigofera linifolia; antidiabetic; antioxidant; nanoparticles.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
% TFC and TPC in Indigofera linifolia crude extract, Ext+Ch NPs, Ext+PEG NPs, and Ext+LGB NPs.
Figure 2
Figure 2
HPLC-UV chromatogram of the crude extract of Indigofera linifolia recorded at 320 nm.
Figure 3
Figure 3
GC chromatogram of Indigofera linifolia leaves extract.
Figure 4
Figure 4
GC–MS chromatograms of detected compounds: (A) 2,6,10-Trimethylpentadecane, (B) Phenol,2,4-bis(1,1-dimethylethyl), (C) Isolongifolene,4,5,9,10-dehydro, (D) 9-Methyl-S-octahydroanthracene, (E) 9-Methyl-S-octahydrophenanthrene, (F) á-Himachalenoxide, (G) Longifolenaldehyde, (H) ç-Gurjunenepoxide-(1), (I) Crocetane, (J) Heneicosane, (K) 9-n-Hexylheptadecane, (L) Octadecane, 3-ethyl-5-(2-ethylbutyl), (M) Diisooctyl phthalate, and (N) Mono(2-ethylhexyl) phthalate.
Figure 4
Figure 4
GC–MS chromatograms of detected compounds: (A) 2,6,10-Trimethylpentadecane, (B) Phenol,2,4-bis(1,1-dimethylethyl), (C) Isolongifolene,4,5,9,10-dehydro, (D) 9-Methyl-S-octahydroanthracene, (E) 9-Methyl-S-octahydrophenanthrene, (F) á-Himachalenoxide, (G) Longifolenaldehyde, (H) ç-Gurjunenepoxide-(1), (I) Crocetane, (J) Heneicosane, (K) 9-n-Hexylheptadecane, (L) Octadecane, 3-ethyl-5-(2-ethylbutyl), (M) Diisooctyl phthalate, and (N) Mono(2-ethylhexyl) phthalate.
Figure 5
Figure 5
Chemical structures of the major phytochemical compounds identified in Indigofera linifolia crude extract.
Figure 6
Figure 6
FTIR spectra of PEG (a), chitosan (b), LGB (c), Ext+Ch NPs (d), Ext+PEG NPs (e), Ext+LBG NPs (f), and crude extract (g).
Figure 7
Figure 7
TG thermograms of Ext+Ch NPs (a), Ext+PEG NPs (b), crude extract (c), and Ext+LBG NPs (d).
Figure 8
Figure 8
SEM images of Indigofera linifolia Ext+PEG NPs (A), Ext+Ch NPs (B), and Ext+LGB NPs (C).
See this image and copyright information in PMC

References

    1. Memariani Z., Abbas S.Q., Ul Hassan S.S., Ahmadi A., Chabra A. Naringin and naringenin as anticancer agents and adjuvants in cancer combination therapy: Efficacy and molecular mechanisms of action, a comprehensive narrative review. Pharmacol. Res. 2021;171:105264. doi: 10.1016/j.phrs.2020.105264. - DOI - PubMed
    1. Cordell G.A. Biodiversity and Drug Discovery—A Symbiotic Relationship. Phytochemistry. 2000;55:463–480. doi: 10.1016/S0031-9422(00)00230-2. - DOI - PubMed
    1. Ul Hassan S.S., Anjum K., Abbas S.Q., Akhter N., Shagufta B.I., Shah S.A.A., Tasneem U. Emerging biopharmaceuticals from marine actinobacteria. Environ. Toxicol. Pharmacol. 2017;49:34–47. doi: 10.1016/j.etap.2016.11.015. - DOI - PubMed
    1. Majid M., Farhan A., Asad M.I., Khan M.R., Hassan S.S.U., Haq I.-U., Bungau S. An Extensive Pharmacological Evaluation of New Anti-Cancer Triterpenoid (Nummularic Acid) from Ipomoea batatas through In Vitro, In Silico, and In Vivo Studies. Molecules. 2022;27:2474. doi: 10.3390/molecules27082474. - DOI - PMC - PubMed
    1. Adang A., Hermkens P. The Contribution of Combinatorial Chemistry to Lead Generation an Interim Analysis. Curr. Med. Chem. 2001;8:985–998. doi: 10.2174/0929867013372517. - DOI - PubMed

LinkOut - more resources