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. 2022 Jul 11:10:940197.
doi: 10.3389/fcell.2022.940197. eCollection 2022.

Enhancement of Chromatin and Epigenetic Reprogramming in Porcine SCNT Embryos-Progresses and Perspectives

Werner Giehl Glanzner  1 , Mariana Priotto de Macedo  1 , Karina Gutierrez  1 , Vilceu Bordignon  1
Affiliations

Enhancement of Chromatin and Epigenetic Reprogramming in Porcine SCNT Embryos-Progresses and Perspectives

Werner Giehl Glanzner et al. Front Cell Dev Biol. .

Abstract

Over the last 25 years, cloned animals have been produced by transferring somatic cell nuclei into enucleated oocytes (SCNT) in more than 20 mammalian species. Among domestic animals, pigs are likely the leading species in the number of clones produced by SCNT. The greater interest in pig cloning has two main reasons, its relevance for food production and as its use as a suitable model in biomedical applications. Recognized progress in animal cloning has been attained over time, but the overall efficiency of SCNT in pigs remains very low, based on the rate of healthy, live born piglets following embryo transfer. Accumulating evidence from studies in mice and other species indicate that new strategies for promoting chromatin and epigenetic reprogramming may represent the beginning of a new era for pig cloning.

Keywords: SCNT; chromatin; cloning; embryo development; epigenetics; histone acetylation; histone methylation; pig.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Chromatin and epigenetic reprogramming impacting SCNT efficiency in pigs. In blue, chromatin/epigenetic modifiers that have been previously shown to play a role on development of pig SCNT embryos and cloning efficiency are listed. In red, modifiers that are believed to have a beneficial effect on the development of pig SCNT embryos based on studies in other species are listed. The figure was created with BioRender.com.
See this image and copyright information in PMC

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