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. 2022 Jul 12:13:957473.
doi: 10.3389/fmicb.2022.957473. eCollection 2022.

Cytotoxic Indolocarbazoles From a Marine-Derived Streptomyces Sp. OUCMDZ-5380

Tongxu Cui  1 , Simin Lin  1 , Zizhen Wang  1 , Peng Fu  1   2 , Cong Wang  3 , Weiming Zhu  1   2
Affiliations

Cytotoxic Indolocarbazoles From a Marine-Derived Streptomyces Sp. OUCMDZ-5380

Tongxu Cui et al. Front Microbiol. .

Abstract

Under the guidance of global natural product social molecular networking, three new indolocarbazoles named streptocarbazoles F-H (1-3), along with staurosporine (4) were isolated from the marine-derived Streptomyces sp. OUCMDZ-5380. Structures of streptocarbazoles F-H were, respectively, determined as N-demethyl-N-hexanoylstaurosporine (1), N-demethyl-N-(2-methyl-3-methoxypyridin-4-yl) staurosporine staurosporine (2), and 4-(N-demethylstaurosporine-N-yl)-1,2-dimethyl-3-methoxypyridinium (3) by spectroscopic analysis and electronic circular dichroism comparison with staurosporine. Compared with staurosporine (4), streptocarbazoles F-H (1-3) showed a selective antiproliferation of the acute myeloid leukemia cell line MV4-11 with the IC50 values of 0.81, 0.55, and 1.88 μM, respectively.

Keywords: Streptomyces sp.; antiproliferatory activity; indolocarbazoles; marine microorganism; streptocarbazoles F–H.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Structures 1–4 and the molecular networking of the extract (MeOH layer) from Streptomyces sp. OUCMDZ-5380 (a) and the compound cluster of indolocarbazole family observed in the molecular networking (b).
FIGURE 2
FIGURE 2
1H-1H COSY and HMBC correlations of compounds 1–3.
FIGURE 3
FIGURE 3
Key NOESY correlations of compounds 1–3.
FIGURE 4
FIGURE 4
Experimental ECD spectra of compounds 1–4.
See this image and copyright information in PMC

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