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. 2022 Sep;8(5):1936-1945.
doi: 10.1002/vms3.894. Epub 2022 Jul 29.

TamaFlex™-A novel nutraceutical blend improves lameness and joint functions in working horses

Sandesh Jain  1 , Satyajit G Patil  2 , Gopichand Chinta  2 , KrishnaRaju Venkata Alluri  2
Affiliations

TamaFlex™-A novel nutraceutical blend improves lameness and joint functions in working horses

Sandesh Jain et al. Vet Med Sci. 2022 Sep.

Abstract

Background: Lameness is one of the major causes of reduced physical performance and early retirement in working horses. TamaFlex™ (NXT15906F6) is a standardized synergistic anti-inflammatory botanical formulation containing Tamarindus indica seed extract and Curcuma longa rhizome extract at a 2:1 ratio.

Methods: We conducted a 12-week single-center, randomized, blinded, placebo-controlled trial demonstrating the efficacy of NXT15906F6 in horses with lameness grade 2-4 on the American Association of Equine Practitioners (AAEP) scale. Twenty-two lame horses were supplemented with NXT15906F6 (2.5 gram/day) or placebo over a period of 84 days. Improvement in lameness over placebo was the primary endpoint, and changes in the levels of rheumatoid factor (RF), anti-nuclear antibody (ANA), and anti-cyclic citrullinated peptide (ACC-peptide) in serum, and pro-inflammatory cytokines including interleukin (IL-1β and IL-6), tumor necrosis factor-α (TNF-α) and prostaglandin-E2 (PGE2 ) in serum and synovial fluid were the secondary endpoints.

Results: NXT15906F6 exhibited significant relief from lameness in a time-dependent manner. NXT15906F6 also reduced levels of ANA, PGE2 , IL-1β, TNF-α and IL-6. Moreover, NXT15906F6 supplementation is safe and tolerable in alleviating joint pain in lame horses, and protects the joints from further degradation by reducing pro-inflammatory mediators.

Conclusion: NXT15906F6 significantly reduces the lameness during walking and trotting, leading to an improvement in their joint flexibility, health, and working performances.

Keywords: AAEP scale; ACC peptide; TamaFlex; lameness; laminitis; rheumatoid factor.

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Conflict of interest statement

The authors declare the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: Satyjit G. Patil, Gopichand Chinta, and KrishnaRaju Venkata Alluri are the employees of Laila Nutraceuticals R&D entre, Vijayawada. Sandesh Jain is the employee of Vins Bioproducts Ltd, Hyderabad, Telangana.

Figures

FIGURE 1
FIGURE 1
Consort flow diagram
FIGURE 2
FIGURE 2
Changes in lameness score from baseline to follow‐up visits. Data were analyzed using the Mann‒Whitney U test. n = 10; *p < 0.05 vs. baseline, #p < 0.05 vs. placebo
FIGURE 3
FIGURE 3
Changes in serum biomarkers from baseline to the end of the supplementation. The bar diagram represents the mean ± SD. Data were analyzed using the Mann‒Whitney U test. n = 10; *p < 0.05 vs. baseline, #p < 0.05 vs. placebo. Abbreviations: ANA, anti‐nuclear antibody; ACC peptide, anti‐cyclic citrullinated peptide; IL‐1β, interleukin‐1β; IL‐6, interleukin‐6; PGE2, prostaglandin E2; RF, rheumatoid factor; TNF‐α, tumour necrosis factor‐α
FIGURE 4
FIGURE 4
Changes in synovial fluid biomarkers from baseline to the end of the supplementation. The bar diagram represents the mean ± SD. Data were analyzed using the Mann‒Whitney U test. n = 10; *p < 0.05 vs. baseline, #p < 0.05 vs. placebo. Abbreviations: IL‐1β, interleukin‐1β; IL‐6, interleukin‐6; PGE2, prostaglandin E2; TNF‐α, tumour necrosis factor‐α
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References

    1. Badmaev, V. , Vik, H. , Stohs, S. J. , & Galluzzo, F. (2018). Safety and toxicological evaluation NXT15906F6 (TamaFlex®): A food‐derived botanical composition containing standardized extracts of Tamarindus indica seeds and Curcuma longa rhizomes. Toxicology Research and Application, 2, 1–14. 10.1177/2397847317749240 - DOI
    1. Baxter, G. M. , Stashak, T. S. , & Keegan, K. G. (2020). Examination for lameness. In Baxter G. M. (Eds.), Adams and Stashak's lameness in horses (2nd ed., pp. 67–188). John Wiley & Sons, Inc. Hoboken. 10.1002/9781119276715.ch2 - DOI
    1. Bertuglia, A. , Pagliara, E. , Grego, E. , Ricci, A. , & Brkljaca‐Bottegaro, N. (2016). Pro‐inflammatory cytokines and structural biomarkers are effective to categorize osteoarthritis phenotype and progression in Standardbred racehorses over five years of racing career. BMC Veterinary Research, 12(1), 246. 10.1186/s12917-016-0873-7 - DOI - PMC - PubMed
    1. Broster, C. E. , Burn, C. C. , Barr, A. R. , & Whay, H. R. (2009). The range and prevalence of pathological abnormalities associated with lameness in working horses from developing countries. Equine Veterinary Journal, 41(5), 474–481. 10.2746/042516409x373907 - DOI - PubMed
    1. Contino, E. K. (2018). Management and rehabilitation of joint disease in sport horses. The Veterinary Clinics of North America. Equine Practice, 34(2), 345–358. 10.1016/j.cveq.2018.04.007 - DOI - PubMed

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