Electrospun kaolin-loaded chitosan/PEO nanofibers for rapid hemostasis and accelerated wound healing

Abstract

Development of chitosan-based hemostatic products and their application in wound healing has always been a research hotspot in the field of emergency treatment and biomedicine. In this study, the classic hemostatic chitosan and the most well-known inorganic hemostatic agent-kaolin were tried to combine to form a more excellent dressing. Together with the aid of non-toxic, harmless and good hydrophilic polyethylene oxide, chitosan/polyethylene oxide (PEO)/kaolin nanofiber membranes (CPKs) were prepared by electrospinning technology. Such membranes exhibited adjustable mechanical properties and good biocompatibility. Furthermore, a series of in vitro coagulation experiments proved that CPKs with 10 % ratio of kaolin (CPK10) has excellent hemostatic ability. Especially, in the whole blood coagulation time (WBCT) assay, the hemostatic time of CPK10 (43 ± 1.4 s) was significantly lower than that of chitosan/polyethylene oxide (CPK0) nanofiber membrane (61 ± 2.2 s) and QuikClot® Combat Gauze (55.7 ± 1.2 s). The further rat liver injury test reconfirmed that CPK10 can stop bleeding better and faster compared to other groups. In addition, CPKs could promote back wound healing in rats within 14 days without significant inflammatory response. This safe and effective hemostatic CPKs is expected to be a promising candidate hemostat in pre-hospital medical care.

Keywords: Chitosan; Electrospinning; Hemostasis; Kaolin; Wound healing.