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. 2023 Feb;73(1):42-47.
doi: 10.1016/j.identj.2022.06.028. Epub 2022 Jul 28.

The Prevalence of EBV and KSHV in Odontogenic Lesions

Mohammed Amjed Alsaegh  1 , Okba Mahmoud  2 , Sudhir Rama Varma  2 , Shengrong Zhu  3
Affiliations

The Prevalence of EBV and KSHV in Odontogenic Lesions

Mohammed Amjed Alsaegh et al. Int Dent J. 2023 Feb.

Abstract

Objectives: Odontogenic lesions evolve as a result of altered dental development. This study aimed to evaluate the prevalence and the coinfection of Epstein-Barr virus (EBV) and Kaposi sarcoma-associated herpesvirus (KSHV) in radicular cysts, dentigerous cysts, odontogenic keratocysts, and ameloblastomas.

Methods: Polymerase chain reaction (PCR) was used to analyse 66 cases of odontogenic lesions for the presence of EBV-DNA and KSHV-DNA. These lesions were 15 radicular cysts, 16 dentigerous cysts, 18 odontogenic keratocysts, and 17 ameloblastomas.

Results: EBV-DNA was detected in 24 (36.4%) of the studied samples as follows: 6 samples (40.0%) of radicular cysts, 4 (25.0%) of dentigerous cysts, 10 (55.6 %) of odontogenic keratocysts, and 4 (23.5%) of ameloblastomas (P = .168). KSHV-DNA was found in 16 (24.2%) of the studied samples as follows: 1 sample (6.7%) of radicular cysts, 6 (37.5%) of dentigerous cysts, 8 (44.4 %) of odontogenic keratocysts, and 1 (5.9%) of ameloblastomas (P = .001). Additionally, EBV and KSHV were positively correlated in all studied samples (P = .002).

Conclusions: Both EBV and KSHV are found in odontogenic cysts and ameloblastomas. KSHV and EBV are more prevalent in odontogenic keratocysts than in other studied odontogenic lesions. Further, there is a high prevalence of EBV and KSHV coinfection in odontogenic cysts and ameloblastomas.

Keywords: Ameloblastomas; Dentigerous cysts; EBV; KSHV; Odontogenic keratocysts; Radicular cysts.

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Conflict of interest statement

Conflict of interest None disclosed.

Figures

Fig 1
Fig. 1
The results of conventional polymerase chain reactions for Epstein-Barr virus DNA (A) and Kaposi sarcoma–associated herpesvirus (B) are shown on ethidium bromide-stained agarose gels. M, molecular marker; PC, positive control; NC, negative control. Results are positive in lanes 1 (radicular cyst), 2 (dentigerous cyst), 3 (odontogenic keratocyst), and 4 (ameloblastoma). Negative results for the studied lesions in lane 5.
Fig 2
Fig. 2
Prevalence of Epstein-Barr virus and Kaposi sarcoma–associated herpesvirus in odontogenic lesions. P values were determined by McNemar test.
See this image and copyright information in PMC

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