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. 2022 Mar 26:3:100129.
doi: 10.1016/j.crmicr.2022.100129. eCollection 2022.

A peculiar cell cycle arrest at g2/m stage during the stationary phase of growth in the wine yeast Hanseniaspora vineae

Luisa Vivian Schwarz  1 , Maria Jose Valera  2 , Ana Paula Longaray Delamare  1 , Francisco Carrau  2 , Sergio Echeverrigaray  1
Affiliations

A peculiar cell cycle arrest at g2/m stage during the stationary phase of growth in the wine yeast Hanseniaspora vineae

Luisa Vivian Schwarz et al. Curr Res Microb Sci. .

Abstract

Yeasts of the genus Hanseniaspora gained notoriety in the last years due to their contribution to wine quality, and their loss of several genes, mainly related to DNA repair and cell cycle processes. Based on genomic data from many members of this genus, they have been classified in two well defined clades: the "faster-evolving linage" (FEL) and the "slower-evolving lineage" (SEL). In this context, we had detected that H. vineae exhibited a rapid loss of cell viability in some conditions during the stationary phase compared to H. uvarum and S. cerevisiae. The present work aimed to evaluate the viability and cell cycle progression of representatives of Hanseniaspora species along their growth in an aerobic and discontinuous system. Cell growth, viability and DNA content were determined by turbidity, Trypan Blue staining, and flow cytometry, respectively. Results showed that H. uvarum and H. opuntiae (representing FEL group), and H. osmophila (SEL group) exhibited a typical G1/G0 (1C DNA) arrest during the stationary phase, as S. cerevisiae. Conversely, the three strains studied here of H. vineae (SEL group) arrested at G2/M stages of cell cycle (2C DNA), and lost viability rapidly when enter the stationary phase. These results showed that H. vineae have a unique cell cycle behavior that will contribute as a new eukaryotic model for future studies of genetic determinants of yeast cell cycle control and progression.

Keywords: Cell cycle; Hanseniaspora; Resting stage; Viability.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that cdocould have appeared to influence the work reported in this paper.

Figures

Image, graphical abstract
Graphical abstract
Fig. 1
Fig. 1
Cell density (formula image), percentage of viable cells (⬜), percentage of cells with one copy of DNA content (grey bars), percentage of cells with two copies of DNA content (white bars) and the representative flow cytometry profiles of (A) Saccharomyces cerevisiae, (B) H. opuntiae, (C) H. uvarum, (D) H. osmophila, (E) H. vineae (T02/05AF), (F) H. vineae (T02/19F) and (G) H. vineae (MVF18.4.1) during aerobic growth. The positions of the peak of 1C and 2C DNA are indicated by arrows.
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