Coapplication of Effective Microorganisms and Nanomagnesium Boosts the Agronomic, Physio-Biochemical, Osmolytes, and Antioxidants Defenses Against Salt Stress in Ipomoea batatas

Abstract

The application of bio- and nanofertilizers are undoubtedly opening new sustainable approaches toward enhancing abiotic stress tolerance in crops. In this study, we evaluated the application of effective microorganisms (EMs) of five groups belonging to photosynthetic bacteria, lactic acid bacteria, yeast, actinobacteria, and fermenting fungi combined with magnesium oxide (MgO) nanoparticles (MgO-NP) on the growth and productivity of sweet potato plants grown in salt-affected soils. In two field experiments carried out in 2020 and 2021, we tested the impacts of EMs using two treatments (with vs. without EMs as soil drench) coupled with three foliar applications of MgO-NP (0, 50, and 100 μg ml^-1 of MgO, representing MgO-NP₀, MgO-NP₅₀, and MgO-NP₁₀₀, respectively). In our efforts to investigate the EMs:MgO-NP effects, the performance (growth and yield), nutrient acquisition, and physio-biochemical attributes of sweet potatoes grown in salt-affected soil (7.56 dS m^-1) were assessed. Our results revealed that salinity stress significantly reduced the growth parameters, yield traits, photosynthetic pigment content (chlorophylls a and b, and carotenoids), cell membrane stability, relative water content, and nutrient acquisition of sweet potatoes. However, the EMs⁺ and/or MgO-NP-treated plants showed high tolerance to salt stress, specifically with a relatively superior increase when any of the biostimulants were combined. The application of EMs and/or MgO-NP improved osmotic stress tolerance by increasing the relative water content and membrane integrity. These positive responses owed to increase the osmolytes level (proline, free amino acids, and soluble sugars) and antioxidative compounds (non-enzymatic concentration, enzymatic activities, phenolic acid, and carotenoids). We also noticed that soil salinity significantly increased the Na⁺ content, whereas EMS⁺ and/or MgO-NP-treated plants exhibited lower Na⁺ concentration and increased K⁺ concentration and K⁺/Na⁺ ratio. These improvements contributed to increasing the photosynthetic pigments, growth, and yield under salinity stress. The integrative application of EMs and MgO-NP showed higher efficacy bypassing all single treatments. Our findings indicated the potential of coapplying EMs and MgO-NP for future use in attenuating salt-induced damage beneficially promoting crop performance.

Keywords: abiotic stress; antioxidant; osmoprotectants; salinity; sweet potato; tuber yield.

FIGURE 1

Interactive effect of effective microorganisms (EMs) interacted with foliar application with magnesium oxide (MgO) nanoparticles (MgO-NP) on total soluble sugars (TSS), proteins, free amino acids, proline, and phenolics of sweet potato (Ipomoea batatas L.) as average for both seasons. Different letters on the bars refer to significant differences among means based on Fisher’s least significant difference test at the p < 0.05 level.

FIGURE 2

Interactive effect of effective microorganisms (EMs) interacted with foliar application with magnesium oxide (MgO) nanoparticles (MgO-NP) on enzymatic [e.g., superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), glutathione (GSH) and ascorbate (AsA)] antioxidants activity of sweet potato (Ipomoea batatas L.) as average for both seasons. Different letters on the bars refer to significant differences among means based on Fisher’s least significant difference test at the p < 0.05 level.