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Review
. 2022 Jul 15:10:952510.
doi: 10.3389/fbioe.2022.952510. eCollection 2022.

Recent Progresses in Electrochemical DNA Biosensors for SARS-CoV-2 Detection

Yanqiu Mei  1 , Xiaofeng Lin  1   2 , Chen He  2 , Weijia Zeng  2 , Yan Luo  2 , Chenghao Liu  2 , Zhehao Liu  1   2 , Min Yang  1   2 , Ying Kuang  1   2 , Qitong Huang  1   2
Affiliations
Review

Recent Progresses in Electrochemical DNA Biosensors for SARS-CoV-2 Detection

Yanqiu Mei et al. Front Bioeng Biotechnol. .

Abstract

Coronavirus disease 19 (COVID-19) is still a major public health concern in many nations today. COVID-19 transmission is now controlled mostly through early discovery, isolation, and therapy. Because of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the contributing factor to COVID-19, establishing timely, sensitive, accurate, simple, and budget detection technologies for the SARS-CoV-2 is urgent for epidemic prevention. Recently, several electrochemical DNA biosensors have been developed for the rapid monitoring and detection of SARS-CoV-2. This mini-review examines the latest improvements in the detection of SARS-COV-2 utilizing electrochemical DNA biosensors. Meanwhile, this mini-review summarizes the problems faced by the existing assays and puts an outlook on future trends in the development of new assays for SARS-CoV-2, to provide researchers with a borrowing role in the generation of different assays.

Keywords: COVID-19; SARS-CoV-2; electrochemical DNA biosensors; nucleic acid detection; virus/protein detection.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
(A) TEM image of SARS-CoV-2 (the blue globules) (Bullock and Tamin, 2020). (B) SARS-CoV-2’s typical structure, S, M, E, and N proteins, encapsulates genomic RNA within virus particles (Kirtipal et al., 2020). (C) A detection workflow of SARS-CoV-2 RNA sequences from clinical samples using the electrochemical biosensor for detection of the S and Orf1ab genes (Kashefi-Kheyrabadi et al., 2022). (D) Schematic diagram of an electrochemical biosensing strategy for SARS-CoV-2 detection using CRISPR/Cas13a. (Heo et al., 2022). (E) Schematic diagram of an electrochemical gene sensor for detecting SARS-COV-2 (Cajigas et al., 2022). (F) Diagram of the preparation steps of the sensor for detecting SARS-CoV-2 S protein. (Abrego-Martinez et al., 2022). (G) Schematic diagram of constructing electrochemical sensor based on Au@Pt/MIL-53 (Tian et al., 2021). (H) Strategy and workflow of SARS-COV-2 antigen detection by CRISPR-based electrochemical sensor (Liu et al., 2022).
See this image and copyright information in PMC

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