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. 2022 Jun;28(6):1277-1295.
doi: 10.1007/s12298-022-01201-8. Epub 2022 Jul 1.

Consensus genetic map and QTLs for morphological and agronomical traits in mungbean using DArT markers

Thi Thuy Hang Vu  1   2 , Adrzeij Kilian  3 , Jason Carling  3 , Robert John Lawn  1   4
Affiliations

Consensus genetic map and QTLs for morphological and agronomical traits in mungbean using DArT markers

Thi Thuy Hang Vu et al. Physiol Mol Biol Plants. 2022 Jun.

Abstract

Mungbean is an important but understudied food legume compared with other major grain crops. Genetic studies through development of high-through put markers, linkage map construction and QTL analysis can accelerate and improve the efficiency of mining for genes for breeding in this crop. This study used four mungbean F5 recombinant inbred lines (RILs) from crosses of two wild types (ACC 1, ACC 87) and two cultivars (Berken, Kiloga) and DArT markers to construct individual and consensus linkage maps and to identify QTLs associated with 54 traits in mungbean. The number of polymorphic DArT markers identified among the four RIL populations varied from 1062 to 2013. The individual maps covered the lengths of 629.7-883.5 cM, comprising 672-981 DArT markers and 15-19 linkage groups (LG) with average distance between markers of 0.9-1.2 cM. The consensus map had the total length of 795.3 cM, comprising 1539 DArT markers and resolved 11 LGs with an average inter-marker distance of 0.65 cM. Sixty-two QTLs were identified for 39 traits across 10 LGs of the consensus map. Major QTLs were identified for two special traits, late flowering inherited from ACC 1 (6 QTLs, PVE of 11.2-29.9%) and perenniality inherited from ACC 87 (3 QTLs, PVE of 17.4-22.6%) in separate population analysis. Number of congruent QTLs across four mungbean populations and the consensus map was 18 for 13 traits. These results illustrated the high efficiency of DArT marker application in mungbean genetic dissection and suggested the future potential employment of identified QTLs for mungbean improvement.

Keywords: Consensus map; DArT; Improvement; QTLs; Vigna radiata.

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Conflict of interest statement

Conflict of interestNot applicable.

Figures

Fig. 1
Fig. 1
A genetic linkage map of mungbean derived from F5 RILs from the cross of Berken x ACC 1. The map includes 981 DArT markers. A centiMorgan scale is on the left
Fig. 2
Fig. 2
A consensus genetic linkage map derived from four mungbean F5 RIL populations from the crosses between two cultivars (Berken and Kiloga) and two wild accessions (ACC 1 and ACC 87). The map includes 1539 DArT markers. A centiMorgan scale is on the left
Fig. 3
Fig. 3
Scatter plots showing the extent of correlations among the mungbean consensus genetic map and four component maps. The DArT markers integrated from four mungbean F5 RIL populations derived from crosses between two cultivars (Berken and Kiloga) and two wild accessions (ACC 1 and ACC 87)
Fig. 4
Fig. 4
Partial and a subset of 5 LGs of DArT linkage map showing 14 major QTLs detected in the mungbean consensus map. QTLs are in bold and black. Traits are indicated next to QTLs
See this image and copyright information in PMC

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