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. 2022 Jan-Mar;48(1):68-74.
doi: 10.12865/CHSJ.48.01.10. Epub 2022 Mar 31.

Combination of Pentoxifylline and Ginko Biloba Nephroprotective Effect in Animal Models with Vancomycin-Induced Nephrotoxicity

Romeo Popa  1 , Magdalena Diaconu  1 , Valentin Cirlig  1 , Ciprian Marius Văruţ  2 , Daniel Cosmin Caragea  3 , Răzvan Andrei Codea  4 , Flaviu Alexandru Tabaran  5 , Mara Aurori  6 , Mihai Gafencu  7 , Cristian Corneliu Georgescu  1
Affiliations

Combination of Pentoxifylline and Ginko Biloba Nephroprotective Effect in Animal Models with Vancomycin-Induced Nephrotoxicity

Romeo Popa et al. Curr Health Sci J. 2022 Jan-Mar.

Abstract

Antioxidants have been commonly used in medicine for thousands of years. Clinically, pentoxifylline and Ginkgo biloba have beneficial renal effects. Our study evaluated the nephroprotective effect of Gingko biloba in combination with Pentoxifiln in an experimental model of vancomycin-induced nephrotoxicity. Male Winstar rats were used in 3 groups: CONTROL, VANCO and VANCO+GBI+PTX and each group included 6 rats. Insufficient studies in the literature on the prevention of acute kidney injury by the combination of Ginkgo biloba and pentoxifylline led to the necessity to perform the study. Acute kidney injury was demonstrated by measuring serum values of classical markers such as urea and creatinine but also by measuring the urinary N-acetyl-β-d-glucosaminidase index, a topical marker in modern medicine. The significant decrease of the biochemical parameters in group III (VANCO+GBI+PTX) compared to group II (VANCO) and values similar to group I (CONTROL), demonstrates, the nephroprotective effect of the use in combination of the two substances.

Keywords: Acute kidney injure; Ginko Biloba; Pentoxifylline; Vancomycin-induced nephrotoxicity.

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Conflict of interest statement

The authors declare that they have no conflict of interests.

Figures

Figure 1
Figure 1
The medications used in our study
Figure 2
Figure 2
Blood and urinary biochemical parameters from the 11th day of the study (lot average values), from the CONTROL, VANCO and VANCO+PTX+GBI lots
Figure 3
Figure 3
Serum urea values from the 11th study day (batch average values), within the CONTROL, VANCO, VANCO+PTX+GBI batches
Figure 4
Figure 4
Urinary iNAG values on the 11th study day in the CONTROL, VANCO, VANCO+PTX+GBI lots
Figure 5
Figure 5
Histopathological sections through the kidney from an individual in the CONTROL group. Normal urinary tubules. Control group, x20ColH&E
Figure 6
Figure 6
Histopathological sections through the kidney from an individual in the VANCO group. Urinary tract with cell necrosis and cytoplasmic vacuolations and occupation of the tubular lumen with necrotic detritus ×20H&E
Figure 7
Figure 7
Histopathological sections through the kidney from an individual in the VANCO+PTX+GBI. Proximal twisted tubes with ischemia ×20H&E
See this image and copyright information in PMC

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