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Observational Study
. 2022 Sep 28;107(10):2748-2757.
doi: 10.1210/clinem/dgac462.

DNA Methylation in Gestational Diabetes and its Predictive Value for Postpartum Glucose Disturbances

Mónica Ballesteros  1   2   3 , Pilar Gil-Lluís  4 , Miriam Ejarque  3   5 , Cristina Diaz-Perdigones  3   5 , Laia Martinez-Guasch  1   3   5 , Sonia Fernández-Veledo  3   5 , Joan Vendrell  1   3   5 , Ana Megía  1   3   5
Affiliations
Observational Study

DNA Methylation in Gestational Diabetes and its Predictive Value for Postpartum Glucose Disturbances

Mónica Ballesteros et al. J Clin Endocrinol Metab. .

Abstract

Context: DNA methylation in the diagnosis of gestational diabetes.

Objective: To assess the value of DNA methylation in the diagnosis of gestational diabetes (GDM) and in the prediction of maternal postpartum glucose disturbances.

Methods: Two-stage observational study performed between July 2006 and December 2010, at University Hospital. Forty-eight randomly selected pregnant women formed the discovery cohort (24 with GDM and 24 controls) and 252 pregnant women (94 with GDM and 158 controls) formed the replication cohort. GDM women were re-evaluated 4 years postpartum. The main outcome measures were GDM, type 2 diabetes or prediabetes at 4 years postpartum.

Results: We identified 3 CpG sites related to LINC00917, TRAPPC9, and LEF1 that were differentially methylated in women with GDM and abnormal glucose tolerance; and sites associated with LINC00917 and TRAPPC9 were independently associated with an abnormal glucose tolerance status 4 years postpartum after controlling for clinical variables. Moreover, the site associated with LINC00917 and the combination of the 3 sites had the highest predictive values.

Conclusion: Our results suggest that some of these sites may be implicated in the development of GDM and postpartum abnormal glucose tolerance.

Keywords: DNA methylation; epigenetic; gestational diabetes; postpartum glucose disturbance.

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Figures

Figure 1.
Figure 1.
Flow chart of the population included in the study.
Figure 2.
Figure 2.
Receiver operating characteristic (ROC) curves showing area under the curve (AUC), 95% CI, and P value in assessing clinical parameters. LEF1, LINC 00917, and TRAPP9 methylation and the combination of the 3 methylated sites (methylation profile) as predictors of abnormal glucose tolerance at 4 years postpartum (A) and assessing the combination of clinical parameters (Model 1), Model 1 + LINC00917 (Model 2) and Model 1 + methylation profile (Model 3) as a predictors of abnormal glucose tolerance (B).
See this image and copyright information in PMC

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