Expression of Long Nonencoding Ribonucleic Acid SNHG20 in Colon Cancer Tissue in Its Influences on Chemotherapeutic Sensitivity of Colon Cancer Cells

Abstract

Noncoding RNA (ncRNA) is a kind of RNA that plays a key role in a variety of biological processes, illnesses, and tumours despite the fact that it cannot be translated into proteins. The HT29 colon cancer cell line was utilized to create a 5-FU drug-resistant cell strain (control group), a lentivirus SNHG20 carrier (OE-SNHG20 group), and an SNHG20 shRNA carrier (SNHG20 shRNA carrier group) (SE-SNHG20 group). To determine the expression of cell SNHG20, a real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR) was utilized, and cholecystokinin-octapeptide (CCK-8) was used to detect the difference in 5-FU inhibitory concentration 50. The goal of the study was to see how variations in long nonencoding ribonucleic acid (lncRNA) SNHG20 expression affect colon cancer cell 5-fluorouracil (5-FU) chemotherapeutic sensitivity by collecting colon cancer and normal para cancer tissues and analysing the differences in SNHG20 expression. The ability of cell cladogenesis was tested using platform cladogenesis. Cell apoptosis was detected using flow cytometry. Western blots revealed the presence of protein phosphatidylinositol kinase (PI3K), protein kinase B (AKT), caspase-3, e-cadherin, and matrix metalloproteinase 9 (MMP-9) enzymes. The findings revealed that SNHG20 expression was considerably upregulated (P < 0.05) in colon cancer tissue and 5-FU drug-resistant colon cancer cells. Cell 5-FU IC50, cell cladogenesis, cell survival rate, and MMP-9, P-PI3K, and P-AKT expression were all significantly improved. Cell apoptosis and expressions of E-cadherin and caspase-3, on the other hand, were considerably decreased (P < 0.05). Cell 5-FU IC50, cell cladogenesis, cell survival rate, and the expressions of MMP-9, P-PI3K, and P-AKT were all significantly lower in the SE-SNHG20 group, although cell apoptosis and the expressions of E-cadherin and caspase-3 were significantly higher (P < 0.05). The results revealed that lncRNA SNHG20 could inhibit the chemotherapeutic sensitivity of colon cancer cells to 5-FU by regulating PI3K/AKT pathways. The inhibition of lncRNA SNHG20 expression could promote the apoptosis and proliferation of 5-FU-resistant colon cancer cells.

Figure 1

Comparison of differences in SNHG20 expressions between colon cancer tissue and normal paracancer tissue. The differences between the two groups revealed statistical significance, ^aP < 0.05.

Figure 2

Fluorescent microscopic observation after the transfection of colon cancer cells by slow virus.

Figure 3

lncRNA SNHG20 expression of colon cancer HT29 cells. The comparison with normal group showed ^aP < 0.05. The comparison with control group revealed ^bP < 0.05. The comparison with LV-NC group demonstrated ^cP < 0.05. The comparison with SE-SNHG20 group suggested ^dP < 0.05.

Figure 4

Influences of SNHG20 expression on the chemotherapeutic sensitivity of colon cancer HT29 cell 5-FU. The comparison with control group suggested ^aP < 0.05. The comparison with LV-NC group indicated ^bP < 0.05. The comparison with SE-SNHG20 group revealed ^cP < 0.05.

Figure 5

Influences of SNHG20 expression on the survival rate of drug-resistant colon cancer HT29 cells. The comparison with control group showed ^aP < 0.05. The comparison with LV-NC group indicated ^bP < 0.05. The comparison with SE-SNHG20 group revealed ^cP < 0.05.

Figure 6

Influences of SNHG20 expression on drug-resistant colon cancer HT29 cell clonogenesis. (a) Trypan blue staining observation of plate clones and (b) the counting of cell clonogenesis. The comparison with control group indicated ^aP < 0.05. The comparison with LV-NC group demonstrated ^bP < 0.05. The comparison with SE-SNHG20 group revealed ^cP < 0.05.

Figure 7

Influences of SNHG20 expression on drug-resistant colon cancer HT29 cell apoptosis. (a) Annexin V-FITC/PI staining observation. (b) Flow cytometry detection. (c) Cell apoptosis. The comparison with control group showed ^aP < 0.05. The comparison with LV-NC group suggested ^bP < 0.05. The comparison with SE-SNHG20 group illustrated ^cP < 0.05.

Figure 8

Influences of SNHG20 expression on drug-resistant colon cancer HT29 cell apoptosis protein expressions. (a) Western blot detection. (b) The relative expression of caspase-3. (c) The relative expression of e-cadherin. (d) The relative expression of MMP-9. The comparison with control group showed ^aP < 0.05. The comparison with LV-NC group suggested ^bP < 0.05. The comparison with SE-SNHG20 group demonstrated ^cP < 0.05.

Figure 9

Influences of SNHG20 expression on protein expressions of PI3K/AKT pathways of drug-resistant colon cancer HT29 cells. (a) Western blot detection. (b) The relative expression of PI3K. (c) PI3K phosphorylation level. (d) The relative expression of AKT. (e) AKT phosphorylation level. The comparison with control group showed that ^aP < 0.05. The comparison with LV-NC group suggested ^bP < 0.05. The comparison with SE-SNHG20 group revealed ^cP < 0.05.