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. 2023 Jun;38(6):637-645.
doi: 10.14670/HH-18-505. Epub 2022 Aug 2.

LncRNA UBE2R2-AS1, as prognostic marker, promotes cell proliferation and EMT in prostate cancer

Feng Wang #  1 , Miao Zhao #  2 , Yuehong Jiang  3 , Silong Xia  1 , Dapeng Sun  1 , Dahong Zhou  1 , Zipu Dong  4
Affiliations

LncRNA UBE2R2-AS1, as prognostic marker, promotes cell proliferation and EMT in prostate cancer

Feng Wang et al. Histol Histopathol. 2023 Jun.

Abstract

Background: Long noncoding RNA ubiquitin-conjugating enzyme E2 R2 antisense RNA 1 (UBE2R2-AS1) has been recently reported to participate in the progression of tumors, including glioma and liver cancer. However, the roles of UBE2R2-AS1 in prostate cancer (PC) remained poorly understood.

Methods: The expression of UBE2R2-AS1 was determined in tumor tissues and paired adjacent tissues from PC patients using quantitative reverse transcription PCR analysis. Correlation between UBE2R2-AS1 expression and clinicopathological parameters and overall survival were investigated by Chi-square test and Kaplan-Meier method analysis. The in vitro experiments, including CCK-8 assay, colony formation, flow cytometry and transwell assay were performed to investigate the functional role of UBE2R2-AS1 knockdown or overexpression on PC cell lines (PC-3 and DU145). Related protein expression levels were measured by western blot analysis.

Results: Our data showed that UBE2R2-AS1 expression was significantly upregulated in PC tissues compared with that in adjacent tissues. The high levels of UBE2R2-AS1 were associated with high Gleason score, advanced clinical T stage, lymph node metastasis and poor prognosis. Knockdown of UBE2R2-AS1 suppressed cell proliferation, migration and invasion, induced cell cycle G0/G1 arrest and apoptosis in PC cells, along with decreased expression of PCNA, CDK4, Cyclin D1, Bcl-2, N-cadherin and Vimentin, and increased E-cadherin expression. Overexpression of UBE12R2-AS1 obtained the opposite results in PC cells.

Conclusions: Our findings suggest that UBE2R2-AS1 might be a potential diagnostic and/or therapeutic target in PC.

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