Microcella aerolata sp. nov., isolated from electronic waste-associated bioaerosols

Abstract

A Gram-positive, non-motile, non-spore-forming and short rod-shaped actinomycete strain, designated GA224^T, was isolated from electronic waste-associated bioaerosols. The optimal growth conditions for this isolate, a facultatively anaerobic bacterium, were 37 °C and pH 8.0. The cell-wall peptidoglycan type was B2γ, with 2,4-diaminobutyric acid (DAB) as the diamino acids, while the major menaquinone was MK-12. The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids, unidentified glycolipids and an unidentified lipid. The major cellular fatty acids were anteiso-C_15:0 and iso-C_16:0. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GA224^T fell within the genus Microcella. The draft genome of strain GA224^T comprised 2,495,189 bp with a G + C content of 72.2 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain GA224^T and the type strain of the type species of Microcella species were lower than 95% and 70%, respectively. Based on the phenotypic, chemotaxonomic and genomic data, strain GA224^T represents a novel species, for which the name Microcella aerolata sp. nov. is proposed, with GA224^T as the type strain (= GDMCC 1.2165 ^T = JCM 34462 ^T).

Keywords: 16S rRNA gene sequence; Bioaerosols; Genomic taxonomy; Microcella aerolata.