Brucellosis in small ruminant: seroprevalence, risk factors, and distribution in the southeast of Algeria

Abstract

The impact of brucellosis on public health and economy is unquestionable in developing countries such as the case of Algeria. This study aimed to provide further understanding of epidemiological status of brucellosis in small ruminant flocks in the southeast of Algeria. Therefore, a cross-sectional study was conducted among small ruminant flocks (n = 51) in El Oued district using simple random sampling strategy. The serum samples collected from 612 sheep and goats (sheep = 280, goats = 332) were screened for Brucella antibodies using the Rose Bengal test (RBT) and the indirect enzyme-linked immunosorbent assay (iELISA) in parallel on all the serum samples. The seropositive serum samples of both tests were confirmed with the complement fixation test (CFT). A structured questionnaire regarding animal, herd, and farm management was prepared and completed in parallel to sampling. Association between variables and Brucella spp. seropositivity status of herds was assessed by univariate and multivariate analysis using simple and binary logistic regression. Estimated true herd prevalence was 27.95% (95% CI, 17.18-42.01), and true individual prevalence was 3.98% (95% CI, 2.51-6.03). Seropositive herds were detected in bordering areas and regions with the highest livestock density. Occurrence of abortions in herds (p = 0.03) increased at least five times (5) the odds of being seropositive (OR, 6.25; 95% CI, 1.20-32.46). Poultry presence in farms revealed to be a protective factor (p = 0.01) (OR, 0.11; 95% CI, 0.02-0.61). The high-level seroprevalence quantified in this study in small ruminant flocks reflects the persistent animal infection endemicity and the high risk of human exposure.

Keywords: Algeria; Brucellosis; Goats; Risk factors; Seroprevalence; Sheep.