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. 2022 Jun;13(2):279-282.
doi: 10.30466/vrf.2020.127645.2952. Epub 2022 Jun 15.

Tetracycline resistant genes in Escherichia coli isolated from enteric disease in companion birds

Majid Gholami-Ahangaran  1 , Maziar Haj-Salehi  2 , Maryam Karimi-Dehkordi  1 , Mohammad Javed Ansari  3 , Ola Abdallah Mahdi  4 , Mohammed Abed Jawad  5
Affiliations

Tetracycline resistant genes in Escherichia coli isolated from enteric disease in companion birds

Majid Gholami-Ahangaran et al. Vet Res Forum. 2022 Jun.

Abstract

Anti-microbial resistant genes could be passed to human via the food chain or by direct contact with infected birds. To evaluate tetracycline resistance genes in the feces of companion birds suspected to enteritis, 100 fecal samples were collected from diarrheic companion birds in Isfahan province, Iran. The presence of Escherichia coli was examined by bacteriological, biochemical, and polymerase chain reaction (PCR) tests. The presence of genes associated with resistance to tetracycline (tetA, tetB, tetC, tetD, tetE, tetG, tetK, tetL, tetM, tetO and tetS genes) was examined using a multiplex PCR. The results showed that in enteric birds, 43.00% of fecal samples contained E. coli. In 26 resistant E. coli, 11, 12 and 3 strains contained tetA (42.30%), tetB (46.15) and tetA plus tetB (11.53%) resistant genes, respectively. In conclusion, E. coli isolates from the enteric problem of companion birds contained tetracycline resistant genes that may transfer to human and pose a risk for antibiotic effectiveness in the treatment of infectious diseases in human.

Keywords: Companion birds; Escherichia coli; Tetracycline resistance gene.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1
Fig. 1
Polymerase chain reaction product electrophoresis. C1: 100 bp marker; C2 to C4: 210 bp fragment of tetA gene; C5 to C8: 659 bp fragment of tetB gene
See this image and copyright information in PMC

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