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. 2022 Dec;35(4):233-264.
doi: 10.1007/s00497-022-00447-2. Epub 2022 Aug 3.

Characterization and analysis of the promoter region of monodehydroascorbate reductase 4 (CpMDAR4) in papaya

Dessireé Zerpa-Catanho  1 , Steven J Clough  2   3 , Ray Ming  4
Affiliations

Characterization and analysis of the promoter region of monodehydroascorbate reductase 4 (CpMDAR4) in papaya

Dessireé Zerpa-Catanho et al. Plant Reprod. 2022 Dec.

Abstract

Differential spatial and temporal expression patterns due to regulatory cis-elements and two different isoforms are detected among CpMDAR4 alleles in papaya. The aim of this research was to study the effects of cis-element differences between the X, Y and Yh alleles on the expression of CpMDAR4, a potential candidate gene for sex differentiation in papaya, using a transcriptional reporter system in a model species Arabidopsis thaliana. Possible effects of a retrotransposon insertion in the Y and Yh alleles on the transcription and expression of CpMDAR4 alleles in papaya flowers were also examined. When comparing promoters and cis-regulatory elements among genes in the non-recombining region of the sex chromosomes, paired genes exhibited differences. Our results showed that differences in the promoter sequences of the CpMDAR4 alleles drove the expression of a reporter gene to different flower tissues in Arabidopsis. β-glucuronidase staining analysis of T2 and T3 lines for constructs containing 5' deletions of native Y and Yh allele promoters showed the loss of specific expression of the reporter gene in the anthers, confirming the existence and location of cis-regulatory element POLLEN1LELAT52. The expression analysis of CpMDAR4 alleles in papaya flowers also showed that all alleles are actively expressed in different flower tissues, with the existence of a shorter truncated isoform, with unknown function, for the Y and Yh alleles due to an LTR-RT insertion in the Y and Yh chromosomes. The observed expression patterns in Arabidopsis thaliana flowers and the expression patterns of CpMDAR4 alleles in papaya flowers suggest that MDAR4 might have a role on development of reproductive organs in papaya, and that it constitutes an important candidate for sex differentiation.

Keywords: Carica papaya; Cis-elements; Isoforms; Monodehydroascorbate reductase; Promoter analysis.

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References

    1. Abu El-Heba GA, Hussein GM, Fahmy IF et al (2015) Impact of cis-acting elements’ frequency in transcription activity in dicot and monocot plants. 3 Biotech 5:1007–1019. https://doi.org/10.1007/s13205-015-0305-6 - DOI - PubMed - PMC
    1. Almagro Armenteros JJ, Salvatore M, Emanuelsson O et al (2019) Detecting sequence signals in targeting peptides using deep learning. Life Sci Alliance 2:e201900429. https://doi.org/10.26508/lsa.201900429 - DOI - PubMed - PMC
    1. Andrews S (2016) FastQC a quality control tool for high throughput sequence data
    1. Bailey TL, Machanick P (2012) Inferring direct DNA binding from ChIP-seq. Nucleic Acids Res 40:e128–e128. https://doi.org/10.1093/nar/gks433 - DOI - PubMed - PMC
    1. Bate N, Twell D (1998) Functional architecture of a late pollen promoter: pollen-specific transcription is developmentally regulated by multiple stage-specific and co-dependent activator elements. Plant Mol Biol 37:859–869. https://doi.org/10.1023/A:1006095023050 - DOI - PubMed

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