Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Aug 3;26(3):26.
doi: 10.1007/s00792-022-01275-y.

Halorientalis salina sp. nov., Halorientalis marina sp. nov., Halorientalis litorea sp. nov.: three extremely halophilic archaea isolated from a salt lake and coarse sea salt

Bei-Bei Wang  1 , Ya-Ping Sun  1 , Zhang-Ping Wu  1 , Xi-Wen Zheng  1 , Jing Hou  1 , Heng-Lin Cui  2
Affiliations

Halorientalis salina sp. nov., Halorientalis marina sp. nov., Halorientalis litorea sp. nov.: three extremely halophilic archaea isolated from a salt lake and coarse sea salt

Bei-Bei Wang et al. Extremophiles. .

Abstract

Three halophilic archaeal strains, NEN8T, GDY88T and ZY14T, were isolated from a salt lake in Tibet and coarse sea salt samples from Guangdong and Hebei, China, respectively. These strains formed three separate clades (showing 94.4-95.8% and 87.1-89.4% similarities, respectively) and then clustered with the current Halorientalis members (showing 90.7-97.6% and 87.0-91.2% similarities, respectively), as revealed by phylogenetic analyses based on 16S rRNA and rpoB' genes. The overall genome-related index, average nucleotide identity (ANI), in silico DNA-DNA hybridization (DDH), average amino acid identity (AAI) and the percentage of conserved proteins (POCP) values, among the three strains and members of the genus Halorientalis were 76.0-88.0%, 21.3-37.2%, 69.0-88.3% and 57.7-78.1%, clearly below the threshold values for species demarcation. Strains NEN8T, GDY88T and ZY14T could be distinguished from current Halorientalis species according to differential phenotypic characteristics. The major polar lipids of the three strains were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), sulfated mannosyl glucosyl diether (S-DGD-1) and disulfated mannosyl glucosyl diether (S2-DGD). In addition, mannosyl glucosyl diether (DGD-1) was detected in strain NEN8T and phosphatidic acid (PA), posssulfated galactosyl mannosyl glucosyl diether (S-TGD-1) and sulfated mannosyl glucosyl diether-phosphatidic acid (S-DGD-PA) were observed in strain ZY14T. These results revealed that strains NEN8T (= CGMCC 1.17213T = JCM 34155T), GDY88T (= CGMCC 1.18548T = JCM 34481T) and ZY14T (= CGMCC 1.17178T = JCM 34154T) represent three novel species of the genus Halorientalis, for which the names Halorientalis salina sp. nov., Halorientalis marina sp. nov. and Halorientalis litorea sp. nov. are proposed.

Keywords: Coarse sea salt; Halophilic archaea; Halorientalis litorea sp. nov.; Halorientalis marina sp. nov.; Halorientalis salina sp. nov.; Salt lake.

PubMed Disclaimer

References

    1. Amoozegar MA, Makhdoumi-Kakhki A, Mehrshad M et al (2014) Halorientalispersicus sp. nov., an extremely halophilic archaeon isolated from a salt lake and emended description of the genus Halorientalis. Int J Syst Evol Microbiol 64(Pt 3):940–944. https://doi.org/10.1099/ijs.0.058164-0 - DOI - PubMed
    1. Cho ES, Cha IT, Roh SW, Nam YD et al (2018) Haloplanus rallus sp. nov., a halophilic archaeon isolated from crude solar salt. Int J Syst Evol Microbiol 68(10):3226–3231. https://doi.org/10.1099/ijsem.0.002970 - DOI - PubMed
    1. Cui HL, Dyall-Smith ML (2021) Cultivation of halophilic archaea (class Halobacteria ) from thalassohaline and athalassohaline environments. Mar Life Sci Technol 3:243–251. https://doi.org/10.1007/s42995-020-00087-3 - DOI
    1. Cui HL, Zhou PJ, Oren A, Liu SJ (2009) Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium. Extremophiles 13:31–37. https://doi.org/10.1007/s00792-008-0194-2 - DOI - PubMed
    1. Cui HL, Gao X, Yang X, Xu XW (2010) Halorussus rarus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern. Extremophiles 14:493–499. https://doi.org/10.1007/s00792-010-0329-0 - DOI - PubMed

Supplementary concepts

LinkOut - more resources