Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Dec;53(4):2299-2307.
doi: 10.1007/s42770-022-00795-1. Epub 2022 Aug 3.

Immunopotential of Pasteurella multocida bivalent outer membrane protein-based vaccine entrapped in aluminum hydroxide nanoparticles

Haladhar Pegu  1 , Shantanu Tamuly  2 , Rajeev Kumar Sharma  3 , Probodh Borah  1 , Rita Nath  4
Affiliations

Immunopotential of Pasteurella multocida bivalent outer membrane protein-based vaccine entrapped in aluminum hydroxide nanoparticles

Haladhar Pegu et al. Braz J Microbiol. 2022 Dec.

Abstract

Swine pasteurellosis is one of the most economically important diseases of pig caused by Pasteurella multocida (P. multocida) capsular types A and D. These organisms are commensals and opportunistic pathogens in the upper respiratory tract in pig. In the present study, we extracted whole outer membrane proteins (OMP) from P. multocida capsular types A and D and were mixed together in the ratio of 1:1 forming bivalent outer-membrane proteins. The bivalent OMP was adsorbed onto aluminum hydroxide nanoparticles. The size of aluminum hydroxide nanoparticles adsorbed outer membrane protein was found to be in the range of 125 to 130 nm. We observed that aluminum hydroxide nanoparticles adjuvanted bivalent OMP-based vaccine elicited quicker immune kinetics in terms of IgG response as compared to aluminum hydroxide microparticles adjuvanted bivalent bacterin vaccine against P. multocida capsular type A and D.

Keywords: Aluminum hydroxide; Nanoparticles; Outer membrane protein (OMP); Pasteurella multocida; Vaccine.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Coomassie brilliant blue stained SDS-PAGE (12%) profile of outer membrane proteins of P. multocida of different capsular type along with standard molecular weight marker. Lane M: molecular marker (14–220 kDa); lane AD: Al(OH)3NP-OMP (A + D); lane D: OMP of P. multocida capsular type D; lane A: OMP of P. multocida capsular type A
Fig. 2
Fig. 2
Transmission electron micrograph of Al(OH)3NP-OMP (A + D)
Fig. 3
Fig. 3
Zeta potential of Al(OH)3NP-OMP (A + D) as determined by DLS
Fig. 4
Fig. 4
IgG response of two vaccines viz. aluminum hydroxide nanoparticle + OMP (A + D) with booster dose (NP-OMP-B) and without booster dose (NP-OMP-NB) and bacterin vaccine against P. multocida capsular type A. ns: p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001
Fig. 5
Fig. 5
IgG response of two vaccines viz. aluminum hydroxide nanoparticle + OMP (A + D) with booster dose (NP-OMP-B) and without booster dose (NP-OMP-NB) and bacterin vaccine against P. multocida capsular type D. ns: p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001
Fig. 6
Fig. 6
Survival of mice against the challenge of P. multocida capsular type A (NP-OMP: aluminum hydroxide nanoparticle + OMP (A + D))
Fig. 7
Fig. 7
Survival of mice against the challenge of P. multocida capsular type D (NP-OMP: aluminum hydroxide nanoparticle + OMP (A + D))
Fig. 8
Fig. 8
Pasteurella multocida species-specific PCR for detection of KMT1 gene of 460 bp in Agarose gel electrophoresis along with 100 bp DNA ladder
Fig. 9
Fig. 9
Pasteurella multocida capsular type A specific PCR showing an amplified product of 1044 bp in agarose gel electrophoresis along with 100 bp DNA ladder
Fig. 10
Fig. 10
Pasteurella multocida capsular type D specific PCR showing an amplified product of 657 bp in agarose gel electrophoresis
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Rajkhowa S, Shakuntala I, Pegu SR, Das RK, Das A. Detection of Pasteurella multocida isolates from local pigs of India by polymerase chain reaction and their antibiogram. Trop Anim Health Prod. 2012;44(7):1497–1503. doi: 10.1007/s11250-012-0094-4. - DOI - PubMed
    1. Varte Z, Dutta T, Roychoudhury P, Begum J, Chandra R (2014) Isolation, identification, characterization and antibiogram of Pasteurella multocida isolated from pigs in Mizoram with special reference to progressive atrophic rhinitis. Vet World 7. 10.14202/vetworld.2014.95-99
    1. Pati US, Srivastava SK, Roy SC, More T. Immunogenicity of outer membrane protein of Pasteurella multocida in buffalo calves. Vet Microbiol. 1996;52(3):301–311. doi: 10.1016/S0378-1135(96)00066-1. - DOI - PubMed
    1. Lu YS, Aguila HN, Lai WC, Pakes SP. Antibodies to outer membrane proteins but not to lipopolysaccharide inhibit pulmonary proliferation of Pasteurella multocida in mice. Infect Immun. 1991;59(4):1470–1475. doi: 10.1128/IAI.59.4.1470-1475.1991. - DOI - PMC - PubMed
    1. Tamuly S, Kumar Saxena M, Saxena A, Kumar Mishra R, Jha R. Comparative evaluation of humoral immune response generated by calcium phosphate nanoparticle adjuvanted and saponin-adjuvanted recombinant outer membrane protein 87 (Omp87) of Pasteurella multocida (serotype B:2) in mice. J Nanopharmaceutics Drug Deliv. 2014;2(1):80–86. doi: 10.1166/jnd.2014.1045. - DOI

Substances

LinkOut - more resources