Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Aug 3;18(1):297.
doi: 10.1186/s12917-022-03403-4.

Clinicopathological and molecular studies on cattle naturally infected with lumpy skin diseases in selected districts of Wolaita Zone, Southern Ethiopia

Mesfin Mathewos  1 , Fistum Dulo  2 , Zewdneh Tanga  2 , Melaku Sombo  3
Affiliations

Clinicopathological and molecular studies on cattle naturally infected with lumpy skin diseases in selected districts of Wolaita Zone, Southern Ethiopia

Mesfin Mathewos et al. BMC Vet Res. .

Abstract

Background: Lumpy skin disease is a contagious viral disease of cattle caused by LSDV that results in huge economic losses in the cattle industry. This study characterizes LSDV in cattle through clinicopathological and molecular techniques in selected districts of Wolaita Zone, Southern Ethiopia.

Methods: A crossectional study was conducted from November 2020 to June 2021 using Real-time polymerase chain reaction and Histopathological techniques to confirm LSDV.

Result: This study revealed that the percentage of positivity of cattle for LSDV was 36.2%. Clinically, cattle infected with LSDV revealed fever (39-41 °C), nodular lesions on the skin and mucous membranes, and lymphadenopathy. Histopathologically, affected tissue revealed ballooning degenerations of the epidermis, infiltration of mononuclear inflammatory cells, vasculitis, and intracytoplasmic eosinophilic inclusion bodies. RT-PCR confirmed that DNA extracts from skin biopsies of virus isolates were positive for LSDV.

Conclusion: The present study confirms that LSDV is widely circulating in cattle of selected districts of the Wolaita zone. Thus, effective control measures through regular vaccination and further confirmation of circulating strains of LSDV through detailed molecular analysis should be recommended.

Keywords: Cattle; Histopathology; Real-time polymerase chain reaction; Wolaita zone; “Lumpy skin disease virus (LSDV)”.

PubMed Disclaimer

Conflict of interest statement

There are no competing interests to declare.

Figures

Fig. 1
Fig. 1
Clinical signs and gross lesions of cattle affected by LSD. Distribution of skin nodules through the body surface of a calf (A), swelling of udder accompanied by mastitis (B), enlargement of prefemoral (C), and prescapular lymph node (D), corneal opacity or keratitis (E), and sit fast lesion (F)
Fig. 2
Fig. 2
Histopathology of LSD: H&E.Ballooning degeneration (arrow) and  intracytoplasmic eosinophilic inclusion bodies (arrowhead) 100 X (a). Hydropic degeneration on the epidermis (arrow) and edema on the dermis (arrowhead) 10 X (b). Inset indicates higher magnification of a and b (40X) (c); Eosinophilic intracytoplasmic inclusion bodies on epidermal cells (arrowhead) (d). Characteristic eosinophilic intracytoplasmic inclusion bodies in follicular cells (arrowhead) (e); Fibrin necrotic vasculitis (f)
Fig. 3
Fig. 3
Amplification fluorescence curves
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Abdallah FM, El Damaty HM, Kotb GF. Sporadic cases of lumpy skin disease among cattle in Sharkia province, Egypt: genetic characterization of lumpy skin disease virus isolates and pathological findings. Vet World. 2018;11:1150. doi: 10.14202/vetworld.2018.1150-1158. - DOI - PMC - PubMed
    1. Abdulqa H, Rahman H, Dyary H, Othman H. Lumpy skin disease. Reprod Immunol. 2016;1:2476–1974.
    1. Abera Z, Degefu H, Gari G. Assessment of distribution and associated risk factors of lumpy skin disease in selected districts of West Wollega Zone, Western Ethiopia. Acad J Anim Dis. 2015;4:130–140.
    1. Abera Z, Degefu H, Gari G, Ayana Z. Review on epidemiology and economic importance of lumpy skin disease. Int J Basic Appl Virology. 2015;4:8–21.
    1. Abutarbush S, Ababneh M, Al Zoubi I, Al Sheyab O, Al Zoubi M, Alekish M, Al Gharabat R. Lumpy skin disease in j ordan: disease emergence, clinical signs, complications and preliminary-associated economic losses. Transbound Emerg Dis. 2015;62:549–554. doi: 10.1111/tbed.12177. - DOI - PubMed

LinkOut - more resources