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Comment
. 2022 Aug 4;7(1):271.
doi: 10.1038/s41392-022-01139-z.

Structural insights into Cas9 mismatch: promising for development of high-fidelity Cas9 variants

Honghai Tang  1   2   3 , Daqi Wang  1   2   3 , Yilai Shu  4   5   6
Affiliations
Comment

Structural insights into Cas9 mismatch: promising for development of high-fidelity Cas9 variants

Honghai Tang et al. Signal Transduct Target Ther. .
No abstract available

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Structures of CRISPR-Cas9 at different states. a Domains of the Cas9-sgRNA-DNA ternary complex are marked by different colors. Cas9 contains 1368 amino acids. b The structures are determined by X-ray diffraction or Cryo-electron microscopy, including Cas9 monomer (PDB ID: 4CMP), Cas9-sgRNA binary complex (PDB ID: 4ZT0), and Cas9-sgRNA-DNA ternary complexes (PDB IDs: 4OO8, 4UN3, 5F9R, 5Y36)
See this image and copyright information in PMC

Comment on

  • Structural basis for mismatch surveillance by CRISPR-Cas9.
    Bravo JPK, Liu MS, Hibshman GN, Dangerfield TL, Jung K, McCool RS, Johnson KA, Taylor DW. Bravo JPK, et al. Nature. 2022 Mar;603(7900):343-347. doi: 10.1038/s41586-022-04470-1. Epub 2022 Mar 2. Nature. 2022. PMID: 35236982 Free PMC article.

References

    1. Bravo JPK, et al. Structural basis for mismatch surveillance by CRISPR-Cas9. Nature. 2022;603:343–347. doi: 10.1038/s41586-022-04470-1. - DOI - PMC - PubMed
    1. Slaymaker IM, et al. Rationally engineered Cas9 nucleases with improved specificity. Science. 2016;351:84–88. doi: 10.1126/science.aad5227. - DOI - PMC - PubMed
    1. Kleinstiver BP, et al. High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. Nature. 2016;529:490–495. doi: 10.1038/nature16526. - DOI - PMC - PubMed
    1. Zhu X, et al. Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9. Nat. Struct. Mol. Biol. 2019;26:679–685. doi: 10.1038/s41594-019-0258-2. - DOI - PMC - PubMed
    1. Jiang F, Doudna JA. CRISPR-Cas9 structures and mechanisms. Annu Rev. Biophys. 2017;46:505–529. doi: 10.1146/annurev-biophys-062215-010822. - DOI - PubMed

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