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. 2022 Aug 4;22(1):136.
doi: 10.1186/s12880-022-00867-6.

Differences in brain activity between normal and diabetic rats under isoflurane anesthesia: a resting-state functional MRI study

Sheng-Min Huang #  1 , Chun-Yi Wu #  2 , Yu-Hsin Lin  3   4 , Hsin-Hua Hsieh  2 , Hui-Chieh Yang  5 , Shao-Chieh Chiu  6 , Shin-Lei Peng  7   8
Affiliations

Differences in brain activity between normal and diabetic rats under isoflurane anesthesia: a resting-state functional MRI study

Sheng-Min Huang et al. BMC Med Imaging. .

Abstract

Background: Altered neural activity based on the fractional amplitude of low-frequency fluctuations (fALFF) has been reported in patients with diabetes. However, whether fALFF can differentiate healthy controls from diabetic animals under anesthesia remains unclear. The study aimed to elucidate the changes in fALFF in a rat model of diabetes under isoflurane anesthesia.

Methods: The first group of rats (n = 5) received a single intraperitoneal injection of 70 mg/kg streptozotocin (STZ) to cause the development of diabetes. The second group of rats (n = 7) received a single intraperitoneal injection of the same volume of solvent. Resting-state functional magnetic resonance imaging was used to assess brain activity at 4 weeks after STZ or solvent administration.

Results: Compared to the healthy control animals, rats with diabetes showed significantly decreased fALFF in various brain regions, including the cingulate cortex, somatosensory cortex, insula, and striatum (all P < 0.05). The decreased fALFF suggests the aberrant neural activities in the diabetic rats. No regions were detected in which the control group had a lower fALFF than that in the diabetes group.

Conclusions: The results of this study demonstrated that the fALFF could be used to differentiate healthy controls from diabetic animals, providing meaningful information regarding the neurological pathophysiology of diabetes in animal models.

Keywords: Anesthesia; Hyperglycemia; Neural; Streptozotocin (STZ); fALFF.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Weekly a weight and b plasma glucose level measurements for animals in the control and diabetes groups. *The difference between the two groups is statistically significant
Fig. 2
Fig. 2
Power spectral density (PSD) in different brain regions between control and diabetes groups. The shaded regions indicate the frequency range (0.01–0.1 Hz) for calculating fractional amplitude of low-frequency fluctuation. SD, standard deviation within each group; a.u., arbitrary unit
Fig. 3
Fig. 3
Fractional amplitude of low-frequency fluctuation (fALFF) maps in the control and diabetes subgroups. The data of animals from each group were averaged for display, and six representative brain sections are shown. EPI, echo-planar imaging
Fig. 4
Fig. 4
Mean fractional amplitude of low-frequency fluctuation (fALFF) of the regions of interest averaged across rats in the control and diabetes groups. *P < 0.05; a.u.: arbitrary unit
See this image and copyright information in PMC

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