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. 2022 Sep;30(9):1796-1805.
doi: 10.1002/oby.23527. Epub 2022 Aug 4.

Validation of energy expenditure and macronutrient oxidation measured by two new whole-room indirect calorimeters

Rebecca Dörner  1 , Franziska A Hägele  1 , Jana Koop  1 , Russell Rising  2 , Thomas Foerster  3 , Thomas Olsen  4 , Mario Hasler  5 , Manfred J Müller  1 , Anja Bosy-Westphal  1
Affiliations

Validation of energy expenditure and macronutrient oxidation measured by two new whole-room indirect calorimeters

Rebecca Dörner et al. Obesity (Silver Spring). 2022 Sep.

Abstract

Objective: The aim of this study was to validate two new whole-room indirfect calorimeters according to Room Indirect Calorimetry Operating and Reporting Standards (RICORS 1.0).

Methods: For technical validation, 16 propane combustion tests were performed to determine accuracy and precision of energy expenditure (EE) and ventilation rates of oxygen (VO2 ), carbon dioxide (VCO2 ), and respiratory exchange ratio (VCO2 /VO2 ). For biological validation, eight participants (mean [SD], age 24.1 [2.5] years; BMI 24.3 [3.1] kg/m2 ) underwent four 24-hour protocols under highly standardized conditions: (1) isocaloric sedentary, (2) fasting sedentary, (3) isocaloric active, and (4) fasting active. Reliability (coefficients of variation [CV]) and minimal detectable changes (MDC) were calculated for 24-hour EE, sleeping metabolic rate (SMR), physical activity energy expenditure (PAEE), thermic effect of food (TEF), and macronutrient oxidation rates.

Results: Technical validation showed high reliability and recovery rates for VO2 (0.75% and 100.8%, respectively), VCO2 (0.49% and 100.6%), and EE (0.54% and 98.2%). Biological validation revealed CV and MDC for active conditions of 1.4% and 4.3% for 24-hour EE, 1.7% and 5.9% for SMR, and 30.2% and 38.4% for TEF, as well as 5.8% and 10.5% for PAEE, respectively. Mean CV and MDC for macronutrient oxidation rates were 9.9% and 22.9%, respectively.

Conclusions: The precision of 24-hour EE and SMR was high, whereas it was lower for PAEE and poor for TEF.

Trial registration: ClinicalTrials.gov NCT04490226.

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References

REFERENCES

    1. Ravussin E, Beyl RA, Poggiogalle E, Hsia DS, Peterson CM. Early time-restricted feeding reduces appetite and increases fat oxidation but does not affect energy expenditure in humans. Obesity (Silver Spring). 2019;27:1244-1254.
    1. Carnero EA, Bock CP, Distefano G, et al. Twenty-four hour assessments of substrate oxidation reveal differences in metabolic flexibility in type 2 diabetes that are improved with aerobic training. Diabetologia. 2021;64:2322-2333.
    1. Nas A, Büsing F, Hägele FA, Hasler M, Müller MJ, Bosy-Westphal A. Impact of energy turnover on fat balance in healthy young men during energy balance, energetic restriction and overfeeding. Br J Nutr. 2020;123:30-40.
    1. Nas A, Mirza N, Hägele F, et al. Impact of breakfast skipping compared with dinner skipping on regulation of energy balance and metabolic risk. Am J Clin Nutr. 2017;105:1351-1361.
    1. Bush NC, Resuehr HES, Goree LL, et al. A high-fat compared with a high-carbohydrate breakfast enhances 24-hour fat oxidation in older adults. J Nutr. 2018;148:220-226.

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