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. 2022 Jun;15(6):768-771.
doi: 10.25122/jml-2021-0335.

Cloning & expression of SAK enzyme from Staphylococcus aureus in E. coli BL21-CodonPlus

Arafat Muttar  1 , Intesar Tarik Numan  2
Affiliations

Cloning & expression of SAK enzyme from Staphylococcus aureus in E. coli BL21-CodonPlus

Arafat Muttar et al. J Med Life. 2022 Jun.

Abstract

Staphylokinase (SAK), also known as staphylococcal fibrinolysin, is a protein with a molecular mass of about 15 kDa produced by Staphylococcus aureus. Staphylokinase is synthesized in the late exponential phase, similar to streptokinase. The current study identified and predicted the protein SAK from Staphylococcus aureus. SAK is a fibrinolytic enzyme of the third generation that acts as an indirect activator of plasminogen. The current study cloned and expressed SAK protein isolated from Staphylococcus aureus and used in the form of a grid for enhancement of SAK Catalyst with PCR, disengagement, and change into articulation vector PET24b(+). The recombinant plasmid was changed into E. coli strain BL21 (codon additionally to 440) acceptance with isopropyl β-D-1-thiogalactopyranoside (IPTG).

Keywords: 15 kDa; BL21-CodonPlus; E. coli; IPTG – isopropyl β-D-1-thiogalactopyranoside; Plasmid pET24b(+); SAK – Staphylokinase; Staphylokinase (SAK); r-SAK – recombinant staphylokinase.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
SAK Enzyme isolated from Staphylococcus aureus PCR in gel electrophoresis.
Figure 2
Figure 2
Transformation of expression host with recombinant plasmid (pET-SAK) in gel electrophoresis.
Figure 3
Figure 3
pET-SAK PCR in gel electrophoresis.
Figure 4
Figure 4
Purification protein and loading in SDS-page.
See this image and copyright information in PMC

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