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Review
. 2022 Jul 14:28:1610342.
doi: 10.3389/pore.2022.1610342. eCollection 2022.

A Detailed Overview About the Single-Cell Analyses of Solid Tumors Focusing on Colorectal Cancer

William J Kothalawala  1 , Barbara K Barták  1 , Zsófia B Nagy  1 , Sára Zsigrai  1 , Krisztina A Szigeti  1 , Gábor Valcz  1   2 , István Takács  1 , Alexandra Kalmár  1   2 , Béla Molnár  1   2
Affiliations
Review

A Detailed Overview About the Single-Cell Analyses of Solid Tumors Focusing on Colorectal Cancer

William J Kothalawala et al. Pathol Oncol Res. .

Abstract

In recent years, the evolution of the molecular biological technical background led to the widespread application of single-cell sequencing, a versatile tool particularly useful in the investigation of tumor heterogeneity. Even 10 years ago the comprehensive characterization of colorectal cancers by The Cancer Genome Atlas was based on measurements of bulk samples. Nowadays, with single-cell approaches, tumor heterogeneity, the tumor microenvironment, and the interplay between tumor cells and their surroundings can be described in unprecedented detail. In this review article we aimed to emphasize the importance of single-cell analyses by presenting tumor heterogeneity and the limitations of conventional investigational approaches, followed by an overview of the whole single-cell analytic workflow from sample isolation to amplification, sequencing and bioinformatic analysis and a review of recent literature regarding the single-cell analysis of colorectal cancers.

Keywords: bioinformatics; colorectal cancer; heterogeneity; multi-omics; single-cell sequencing.

PubMed Disclaimer

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Summary of the workflow of single cell isolation and downstream analyses.
See this image and copyright information in PMC

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