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. 2022 Jul 26:2022:5873479.
doi: 10.1155/2022/5873479. eCollection 2022.

Effects of mir-195 Targeted Regulation of JAK2 on Proliferation, Invasion, and Apoptosis of Gastric Cancer Cells

Yu Zhang  1 , Kun Zhuang  1 , Shanshan Yuan  1 , Wangli Si  1 , Yijun Li  1 , Jun Zhang  1 , Jiaming Liu  1
Affiliations

Effects of mir-195 Targeted Regulation of JAK2 on Proliferation, Invasion, and Apoptosis of Gastric Cancer Cells

Yu Zhang et al. Comput Math Methods Med. .

Retraction in

Abstract

Background: Overexpression of miR-195 can make gastric cancer cells stay in G1/G2 phase. miR-195 has been shown to inhibit gastric cancer cell replication and accelerate cell death by targeting JAK2. However, the relationship between miR-195, JAK2, and gastric cancer is not clear.

Objective: To observe the effect of mir-195 regulated by JAK2 on the growth, invasion, and death of gastric cancer cells.

Methods: MGC803 and NCI gastric N87 cells were introduced into the negative control sequences of miR-195 and RNA, respectively. To detect the expression of miR-195 in cells, to detect the effect of miR-195 on mitosis and proliferation of tumor cells, to analyze the effect of miR-195 on cell invasion and metastasis, and to detect the regulation of miR-195 on JAK2 expression.

Results: The level of miR-195 in miR-195-MIMICS group was significantly higher than that in miR-NC group. The cell survival rate of miR-195 mimic group was lower than that of miR-NC group (P < 0.05). Compared with miR-NC group, the number of cells in G1 phase increased, the cells in G2 phase and S phase decreased, and the proportion of cells in G2 and S phase decreased in miR-195 mimic group. The scratch distance of miR-195 simulator group was larger than that of control group. The number of invasive cells in the miR-195 mimic group was significantly lower than that in the control group. The expression of JAK2 protein in miR-195 mimic group was lower than that in miR-NC group. There was a significant negative correlation between the expression level of miR-195 and JAK2 (rhabdomile 0.326 and record 0.00). There are continuous interaction fragments between JAK2 and miR-195. The luciferase activity of miR-195 mimic and wild type JAK2 sequence expression vector was significantly lower than that of wild type JAK2 sequence expression vector.

Conclusion: miR-195 may inhibit the occurrence, metastasis, and invasion of gastric tumor by downregulating the expression of JAK2. miR-195/JAK2 may be a new molecular target for the treatment of gastrointestinal tumors.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
Note: the relative level of miR-195 mRNA in mi R-NC group was compared with that in miR-195 mimics group (P < 0.05). ∗ indicates that the difference is statistically significant (P < 0.05).
Figure 2
Figure 2
Note: (a) MTT test detected the capability of cell proliferation after miR-195 mimics transfection. (b, c) The apoptosis after miR-195 mimics transfection by flow cytometry, the ratio of G1/S and G2/S phase cells. The apoptosis rate of mi R-NC group was remarkably greater than miR-195mimics group. ∗Compared with miR-195 mimics group, P < 0.05.
Figure 3
Figure 3
Note: (a) scratch distance of each group (100×). (b) The number of cells attached to the bottom of the membrane in each group (100×). ∗Compared with miR-NC group, P < 0.05.
Figure 4
Figure 4
Note: (a) miR-195 has negative correlation with JAK2. (b) The content of JAK2 protein in cells of each group. ∗Compared with miR-NC group, P < 0.05.
Figure 5
Figure 5
Note: (a) double luciferase report results; prediction map of targeted binding sites of (b) miR-195 and JAK2. ∗∗Compared with JAK2 5WT group, P < 0.01.
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