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Meta-Analysis
. 2022 Aug 6;15(1):285.
doi: 10.1186/s13071-022-05397-2.

Prevalence of Plasmodium spp. in Anopheles mosquitoes in Thailand: a systematic review and meta-analysis

Chutipong Sukkanon  1 , Frederick Ramirez Masangkay  2 , Wanida Mala  1 , Kwuntida Uthaisar Kotepui  1 , Polrat Wilairatana  3 , Theeraphap Chareonviriyaphap  4   5 , Manas Kotepui  6
Affiliations
Meta-Analysis

Prevalence of Plasmodium spp. in Anopheles mosquitoes in Thailand: a systematic review and meta-analysis

Chutipong Sukkanon et al. Parasit Vectors. .

Abstract

Background: The entomological inoculation rate (EIR) is one of the key indices used to evaluate malaria transmission and vector control interventions. One of the components of the EIR is the sporozoite rate in Anopheles vectors. A systematic review and meta-analysis was performed to identify the prevalence of Plasmodium spp. in field-collected Anopheles species across Thailand.

Methods: This systematic review was registered under the PROSPERO number CRD42021297255. Studies that focused on the identification of Plasmodium spp. in Anopheles mosquitoes were identified from the electronic databases PubMed, Web of Science, and Scopus. The quality of the identified studies was determined using the Strengthening the Reporting of Observational Studies in Epidemiology approach. The proportion of Anopheles mosquitoes collected, Anopheles vectors for Plasmodium species, and specificity of Anopheles vectors for Plasmodium species were analyzed. The pooled prevalence of Plasmodium species among the primary vectors (Anopheles dirus, Anopheles minimus, and Anopheles maculatus) was estimated using the random-effects model.

Results: Of the 1113 studies identified, 31 were included in the syntheses. Of the 100,910 Anopheles mosquitoes identified for species and sibling species, An. minimus (40.16%), An. maculatus (16.59%), and Anopheles epiroticus (9.18%) were the most prevalent Anopheles species. Of the 123,286 Anopheles mosquitoes identified, 566 (0.46%) were positive for Plasmodium species. The highest proportions of Plasmodium species were identified in Anopheles hodgkini (2/6, 33.3%), Anopheles nigerrimus (2/24, 8.33%), Anopheles balabacensis (4/84, 4.76%), An. dirus (114/4956, 2.3%), Anopheles annularis (16/852, 1.88%), Anopheles kochi (8/519, 1.54%), Anopheles vagus (3/215, 1.4%), and Anopheles baimaii (1/86, 1.16%). The pooled prevalence of Plasmodium species identified in the main Anopheles vectors was 0.4% of that of Plasmodium species identified in An. dirus was 2.1%, that of Plasmodium species identified in An. minimus was 0.4%, and that of Plasmodium species identified in An. maculatus was 0.4%.

Conclusions: We found a low prevalence of Plasmodium infection in Anopheles mosquitoes across Thailand. Therefore, the use of EIR to determine the impact of vector control intervention on malaria parasite transmission and elimination in Thailand must be undertaken with caution, as a large number of Anopheles specimens may be required.

Keywords: Anopheles; Meta-analysis; Plasmodium; Thailand.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Study flow diagram
Fig. 2
Fig. 2
Geographic distribution of mosquito identification studies with isolated malaria-causing Plasmodium spp. in Thailand. Map of Thailand (Thailand location map.svg) was sourced license-free from Wikimedia commons: https://commons.wikimedia.org/w/index.php?search=thailand+map&title=Special:MediaSearch&go=Go&type=image
Fig. 3
Fig. 3
The pooled prevalence of Plasmodium spp. in all vectors. ES, prevalence estimate; 95% CI: confidence interval
Fig. 4
Fig. 4
The pooled prevalence of Plasmodium spp. in primary vectors (An. minimus, An. maculatus, An. dirus). ES, prevalence estimate; 95% CI: confidence interval
Fig. 5
Fig. 5
The pooled prevalence of Plasmodium spp. in An. dirus. ES, prevalence estimate; 95% CI: confidence interval
Fig. 6
Fig. 6
The pooled prevalence of Plasmodium spp. in An. minimus. ES, prevalence estimate; 95% CI: confidence interval
Fig. 7
Fig. 7
The pooled prevalence of Plasmodium spp. in An. maculatus. ES, prevalence estimate; 95% CI: confidence interval
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