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. 2022 Aug 2;6(8):e758.
doi: 10.1097/HS9.0000000000000758. eCollection 2022 Aug.

Retrospective Longitudinal Monitoring of Multiple Myeloma Patients by Mass Spectrometry Using Archived Serum Protein Electrophoresis Gels and De Novo Sequence Analysis

Somayya Noori  1 , Marina Zajec  1   2 , Henk Russcher  2 , Andrei N Tintu  2 , Annemiek Broijl  3 , Joannes F M Jacobs  4 , Theo M Luider  1 , Yolanda B de Rijke  2 , Martijn M vanDuijn  1
Affiliations

Retrospective Longitudinal Monitoring of Multiple Myeloma Patients by Mass Spectrometry Using Archived Serum Protein Electrophoresis Gels and De Novo Sequence Analysis

Somayya Noori et al. Hemasphere. .
No abstract available

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Figures

Figure 1.
Figure 1.
SPEP gels and de novo sequencing. Two representative SPEP gels are shown from patient 7, 1 positive for M-protein, the other negative. The gel band that was excised for mass spectrometry analysis has been indicated. The result of de novo sequence analysis on the first gel sample is shown, indicating fragment ion peaks associated with the amino acid sequence of the peptide and mass errors. Other peptides in Suppl. Figure S1. SPEP = serum protein electrophoresis.
Figure 2.
Figure 2.
Comparison of M-protein monitoring by routine electrophoretic techniques (serum protein electrophoresis and immunofixation electrophoresis) and the mass spectrometry assay. Routine M-protein monitoring is shown in orange. M-protein concentration ≤5 g/L is plotted at 5 g/L, SPEP data for patient 1 includes interference from the β region. MS signals are indicated in arbitrary units and shown in blue for heavy chain peptides, and in red for the light chain. Empty symbols indicate that the M-protein/M-protein peptide was not detectable and were plotted at the level of the lowest positive sample. Patient treatment periods are shown in yellow, maintenance periods are plotted at a lower height. Bone marrow flow cytometry data are plotted as a green cross (MRD negative) or as a red plus (MRD positive). Panels P1–P9 shows the 9 patients and the panel marked Ref represents a dilution series based on the reference patient sample. MRD = minimal residual disease.
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