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. 2022 Aug 1;7(8):1424-1426.
doi: 10.1080/23802359.2022.2107458. eCollection 2022.

Nanopore sequencing of chloroplast genome of Scapania undulata (L.) Dumort., 1835 (Scapaniaceae, Jungermanniales)

Kamil Ciborowski  1 , Bartosz Skierkowski  1 , Karolina Żukowska  1 , Katarzyna Krawczyk  1 , Jakub Sawicki  1
Affiliations

Nanopore sequencing of chloroplast genome of Scapania undulata (L.) Dumort., 1835 (Scapaniaceae, Jungermanniales)

Kamil Ciborowski et al. Mitochondrial DNA B Resour. .

Abstract

We performed long-read sequencing of the Scapania undulata (L.) Dumort. chloroplast genome with Oxford Nanopore Technology using improved flow cells (10.3). S. undulata is a leafy liverwort living on rocks in or near water in mountains. The size of the complete plastome sequence is 117,651 bp long, the large single copy (LSC) region is 80,606 bp long, the small single copy (SSC) region is 19,401 bp long. The nanopore sequencing allowed for the distinction of inverted repeats A and B (IRA, IRB) which were not identical. Guanine cytosine (GC) ratio is 33.7% for the complete chloroplast genome, for LSC and SSC are 31.7% and 30.6%. The phylogeny reconstruction shows that Scapania undulata is the sister to S. ciliata and S. griffithii.

Keywords: Liverwort; Scapania; chloroplast genome; nanopore.

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Conflict of interest statement

No potential conflict of interest was reported by the author(s).

Figures

Figure 1.
Figure 1.
Phylogenetic relationships of liverworts based on plastome sequences obtained using Bayesian inference. The posterior probability values are given at nodes.
See this image and copyright information in PMC

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