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. 2022 Nov;133(5):2954-2965.
doi: 10.1111/jam.15750. Epub 2022 Aug 8.

Enhancement of antibacterial and growth-promoting effects of Paenibacillus polymyxa by optimizing its fermentation process

Shoude Liu  1   2 , Huamei Liu  2 , Li Zhou  2 , Zhiguo Cheng  2 , Jun Wan  2 , Yu Pan  2 , Guang Xu  2 , Fang Huang  2 , Meng Wang  2 , Yuanyuan Xiong  2 , Guoyuan Hu  1
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Enhancement of antibacterial and growth-promoting effects of Paenibacillus polymyxa by optimizing its fermentation process

Shoude Liu et al. J Appl Microbiol. 2022 Nov.

Abstract

Aims: We aimed to enhance the antibacterial and growth-promoting effects of Paenibacillus polymyxa by improving the yield of spores, lipopeptides and indole-3-acetic acid (IAA) in the fermentation process.

Methods and results: Through medium optimization by the response surface method and feeding fermentation, the number of spores reached 2.37 × 109 cfu ml-1 with an increase of 38%, the content of lipopeptides reached 60.8 mg L-1 with an increase of 89%, and the content of IAA reached 24.3 mg L-1 with an increase of 176%, respectively, comparing with the original (un-optimized) culture conditions. The fermentation culture of P. polymyxa from the optimized medium and feeding fermentation resulted in higher colonization of P. polymyxa in soils than that from the original culture during the 49 days for testing. Comparing with the supernatant of the original culture, the supernatant of the P. polymyxa culture from the optimized medium and feeding fermentation showed enhanced antibacterial effects and plant growth-promoting effects. The enhanced antibacterial effect was shown as the increase of the inhibition zone by 59%, 45% and 26% against Ralstonia solanacearum, Erwinia carotovora and Xanthomonas campestris. The enhanced growth-promoting effects on tomato and strawberry plants were the increase of plant height by 47% and 5%, root length by 23% and 15% and root weight by 65% and 110%.

Conclusions: The combination of medium optimization and feeding fermentation effectively improved the yield of spores, lipopeptides and IAA. Lipopeptides and IAA lead to enhanced antibacterial and plant growth-promoting effects of the P. polymyxa product.

Significance and impact of this study: The optimized fermentation method significantly improved the yield of spores, lipopeptides and IAA, thus providing theoretical and technical support for enhancing the antibacterial and growth-promoting effects of P. polymyxa products in agriculture.

Keywords: Paenibacillus polymyxa; IAA; antibacterial; fermentation optimization; growth promotion; lipopeptides; spores.

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