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. 2022 Aug 9;38(10):179.
doi: 10.1007/s11274-022-03363-9.

Assessment of the antioxidant, antimicrobial and antibiofilm activities of essential oils for potential application of active chitosan films in food preservation

Anouar Mouhoub  1 , Amine Guendouz  2 , Abdeljalil Belkamel  1 , Zainab El Alaoui Talibi  1 , Saad Ibnsouda Koraichi  3 , Cherkaoui El Modafar  1 , Cédric Delattre  4   5
Affiliations

Assessment of the antioxidant, antimicrobial and antibiofilm activities of essential oils for potential application of active chitosan films in food preservation

Anouar Mouhoub et al. World J Microbiol Biotechnol. .

Abstract

In the food industry, the development of microbial biofilms is a serious problem that leads to the contamination and deterioration of food products. To overcome that, our aim consists of searching for natural antimicrobial and non-toxic compounds (essential oils EOs), which might be used alone or adsorbed on natural biopolymer films (chitosan). In this work, the antioxidant activity of eight EOs was evaluated by DPPH radical-scavenging method while their antibacterial activity was determined by diffusion on agar and microdilution methods. Among all tested EOs, Eugenia caryophyllus, Cinnamomum zeylanicum Blume and Thymus satureioides Cosson showed high antioxidant activities at the concentration of 25.6 mg/mL, with respective values of (86.26%, 81.75%, and 76%), and strong antibacterial activity against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Enterococcus hirae, with (MIC) values ≤ 4 µL/mL. At the concentration of 1 µL/mL, these EOs tested alone, showed values of antibiofilm-forming activity ranging from 79.43 to 99.33% and from 44.18 to 94.17%, when they are adsorbed onto chitosan film. These promising results confirm that these three EOs have a good potential for an eventual application in the food industry, as antimicrobial and antioxidant agents, or as active biodegradable food packaging, if combined with chitosan.

Keywords: Antibacterial activity; Antibiofilm activity; Antioxidant activity; Chitosan; Essential oil.

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References

    1. Aaron SD, Ferris W, Ramotar K, Vandemheen K, Chan F, Saginur R (2002) Single and combination antibiotic susceptibilities of planktonic, adherent, and biofilm-grown Pseudomonas aeruginosa isolates cultured from sputa of adults with cystic fibrosis. J Clin Microbiol 40(11):4172–4179. https://doi.org/10.1128/JCM.40.11.4172-4179.2002 - DOI - PubMed - PMC
    1. Ait-Ouazzou A, Lorán S, Arakrak A, Laglaoui A, Rota C, Herrera A, Pagán R, Conchello P (2012) Evaluation of the chemical composition and antimicrobial activity of Mentha pulegium, Juniperus phoenicea, and Cyperus longus essential oils from Morocco. Food Res Int 45(1):313–319. https://doi.org/10.1016/j.foodres.2011.09.004 - DOI
    1. Akinyemi K, Oluwa O, Omomigbehin E (2006) Antimicrobial activity of crude extracts of three medicinal plants used in south-west Nigerian folk medicine on some food borne bacterial pathogens. Afr J Tradit Complem Altern Med 3(4):13–22. https://doi.org/10.4314/ajtcam.v3i4.31173 - DOI
    1. Alibi S, Selma WB, Ramos-Vivas J, Smach MA, Touati R, Boukadida J, Navas J, Mansour HB (2020) Anti-oxidant, antibacterial, anti-biofilm, and anti-quorum sensing activities of four essential oils against multidrug-resistant bacterial clinical isolates. J Curr Res Transl Med 68(2):59–66. https://doi.org/10.1016/j.retram.2020.01.001 - DOI
    1. Alizadeh Behbahani B, Falah F, Lavi Arab F, Vasiee M, Yazdi Tabatabaee, FJE-bc, medicine a. (2020) Chemical composition and antioxidant, antimicrobial, and antiproliferative activities of Cinnamomum zeylanicum bark essential oil. Evid Based Complem Alternat Med 2020:5190603. https://doi.org/10.1155/2020/5190603 - DOI

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