Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Oct;43(20):2044-2048.
doi: 10.1002/elps.202200057. Epub 2022 Aug 10.

Electrokinetic characterization of hybrid NOA 81-glass microchips: Application to protein microchip electrophoresis with indirect fluorescence detection

Menel Ben Frej  1 , Fanny d'Orlyé  1 , Gerson F Duarte-Junior  1   2 , Wendell K T Coltro  2 , Anne Varenne  1
Affiliations

Electrokinetic characterization of hybrid NOA 81-glass microchips: Application to protein microchip electrophoresis with indirect fluorescence detection

Menel Ben Frej et al. Electrophoresis. 2022 Oct.

Abstract

A low-cost and straightforward hybrid NOA (Norland optical adhesive) 81-glass microchip electrophoresis device was designed and developed for protein separation using indirect fluorescence detection. This new microchip was first characterized in terms of surface charge density via electroosmotic mobility measurement and stability over time. A systematic determination of the electroosmotic mobility (μeo ) over a wide pH range (2-10) and at various ionic strengths (20-50 mM) was developed for the first time via the neutral marker approach in an original simple frontal methodology. The evolution of μeo was proved consistent with the silanol and thiol functions arising from the glass and the NOA materials, respectively. The repeatability and reproducibility of the measurements on different microchips (RSD < 14%) and within 15 days (less than 5% decrease) were successfully demonstrated. The microchip was then applied for the efficient electrophoretic separation of proteins in a zonal mode coupled with indirect fluorescence detection, which is, to our knowledge, the first proof of concept of capillary zone electrophoresis in this hybrid microsystem.

Keywords: Norland 81; electroosmotic mobility; hybrid glass-based chip; indirect fluorescence detection; proteins.

PubMed Disclaimer

References

REFERENCES

    1. Nielsen JB, Hanson RL, Almughamsi HM, Pang C, Fish TR, Woolley AT. Microfluidics: innovations in materials and their fabrication and functionalization. Anal Chem. 2020;92:150-68.
    1. Coltro WKT, Lunte SM, Carrilho E. Comparison of the analytical performance of electrophoresis microchannels fabricated in PDMS, glass, and polyester-toner. Electrophoresis. 2008;29:4928-37.
    1. Rodriguez I, Spicar-Mihalic P, Kuyper CL, Fiorini GS, Chiu DT. Rapid prototyping of glass microchannels. Anal Chim Acta. 2003;496:205-15.
    1. van Assenbergh P, Meinders E, Geraedts J, Dodou D. Nanostructure and microstructure fabrication: from desired properties to suitable processes. Small. 2018;14:1703401.
    1. Zhou W, Dou M, Timilsina SS, Xu F, Li XJ. Recent innovations in cost-effective polymer and paper hybrid microfluidic devices. Lab Chip. 2021;21:2658-83.

Publication types

MeSH terms

LinkOut - more resources