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. 2022 Aug 10;20(1):13.
doi: 10.1186/s12953-022-00195-1.

Tear proteomics analysis of patient suffered from delayed mustard gas keratopathy

Affiliations

Tear proteomics analysis of patient suffered from delayed mustard gas keratopathy

Shahram Parvin et al. Proteome Sci. .

Abstract

Understanding the molecular and cellular mechanisms involved in the pathogenesis of ocular injured induced by mustard gas can help better identify complications and discover appropriate therapies. This study aimed to analyze the proteomics of tears of chemical warfare victims with mustard gas ocular injuries and compare it with healthy individuals. In this case-control research, 10 mustard gas victims with long-term ocular difficulties (Chronic) were included in the patient group, while 10 healthy persons who were age and sex matched to the patients were included in the control group. Schirmer strips were used to collect the tears of the participants. Proteomics experiments were performed using the high-efficiency TMT10X method to evaluate the tear protein profile, and statistical bioinformatics methods were used to identify the differently expressed proteins. 24 proteins had different expressions between the two groups. Among these 24 proteins, 8 proteins had increased expression in veterans' tears, while the remaining 16 proteins had decreased expression. Reactome pathways were used to look at proteins with various expressions, and 13 proteins were found to be engaged in the immune system, 9 of which were effective in the innate immune system, and 5 proteins were effective in the complement cascade. Ocular mustard gas exposure may cause a compromised immune system on the eye's surface, exposing the cornea to external and endogenous infections, and eventually causing corneal opacity and reduced vision.

Keywords: Ocular complications; Proteomics; Sulfur mustard; Tear.

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Conflict of interest statement

Authors have no conflict of interest to declare.

Figures

Fig. 1
Fig. 1
Density plot (left panel) and Box plots (right panel) of post-normalized data showing variability in the TMT-MS data tear
Fig. 2
Fig. 2
Heatmap plot for visualize hierarchical clustering
Fig. 3
Fig. 3
Volcano plot of differentially abundant proteins in the tear

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References

    1. Borak J, Sidell FR. Agents of chemical warfare: sulfur mustard. Ann Emerg Med. 1992;21(3):303–308. - PubMed
    1. Ghabili K, Agutter PS, Ghanei M, Ansarin K, Panahi Y, Shoja MM. Sulfur mustard toxicity: history, chemistry, pharmacokinetics, and pharmacodynamics. Crit Rev Toxicol. 2011;41(5):384–403. - PubMed
    1. Panahi Y, Abdolghaffari AH, Sahebkar A. A review on symptoms, treatments protocols, and proteomic profile in sulfur mustard-exposed victims. J Cell Biochem. 2018;119(1):197–206. - PubMed
    1. Balali-Mood M, Hefazi M. Comparison of early and late toxic effects of sulfur mustard in Iranian veterans. Basic Clin Pharmacol Toxicol. 2006;99(4):273–282. - PubMed
    1. Kehe K, Szinicz L. Medical aspects of Sulphur mustard poisoning. Toxicology. 2005;214(3):198–209. - PubMed

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