Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2527:41-56.
doi: 10.1007/978-1-0716-2485-2_4.

Somatic Embryogenesis in Pines

Ander Castander-Olarieta  1 , Paloma Moncaleán  1 , Itziar A Montalbán  2
Affiliations

Somatic Embryogenesis in Pines

Ander Castander-Olarieta et al. Methods Mol Biol. 2022.

Abstract

Among the different in vitro culture techniques, somatic embryogenesis has been one of the most important developments for plant tissue culture; it has enabled mass propagation and the development of biotechnological tools to enhance the productivity and quality of plantation forestry. This propagation technique together with cryopreservation is the base of multivarietal forestry.The development of somatic embryogenesis in forest trees dates from 1985, and in the last years several studies have focused on the development and optimization of the conifer somatic embryogenesis process to make it more efficient in terms of both the quantity and the characteristics of the plants obtained. However, these advances are not sufficiently refined to be implemented commercially for many Pinus spp. due to the high cost of the process derived from hand labor. Nowadays, trying to add value to the plants produced to compensate the high costs of the process, different studies are being developed in order to obtain Pinus somatic plants with better adaptation to environmental stresses prompted by the current situation of climate change.In this chapter, a summary of the recent somatic embryogenesis systems developed to achieve Pinus spp. high quality plants is presented.

Keywords: Abiotic stress; Conifer; Cryopreservation; Forest Biotechnology; Micropropagation; Somatic Embryos.

PubMed Disclaimer

References

    1. Montalbán IA, De Diego N, Moncaleán P (2011) Testing novel cytokinins for improved in vitro adventitious shoots formation and subsequent ex vitro performance in Pinus radiata. Forestry 84:363–373. https://doi.org/10.1093/forestry/cpr022 - DOI
    1. Alonso P, Moncaleán P, Fernández B et al (2006) An improved micropropagation protocol for stone pine (Pinus pinea L). Ann For Sci 63:1–7. https://doi.org/10.1051/forest:2006071 - DOI
    1. Tereso S, Gonçalves S, Marum L et al (2006) Improved axillary and adventitious bud regeneration from Portuguese genotypes of Pinus pinaster Ait. Propag Ornam Plants 6:24–33
    1. De Diego N, Montalbán IA, Moncaleán P (2011) Improved micropropagation protocol for maritime pine using zygotic embryos. Scan J Forest Res 26:1–10. https://doi.org/10.1080/02827581.2011.559174 - DOI
    1. Moncaleán P, Alonso P, Centeno ML et al (2005) Organogenic response of Pinus pinea cotyledons to hormonal treatments: BA metabolism and cytokinin content. Tree Physyol 25:1–9. https://doi.org/10.1093/treephys/25.1.1 - DOI

Publication types

LinkOut - more resources