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. 2022:2538:75-93.
doi: 10.1007/978-1-0716-2529-3_6.

Structural Information on Bacterial Amyloid and Amyloid-DNA Complex Obtained by Small-Angle Neutron or X-Ray Scattering

Tatsuhito Matsuo  1   2   3 , Véronique Arluison  4   5 , Frank Wien  6 , Judith Peters  7   8   9
Affiliations

Structural Information on Bacterial Amyloid and Amyloid-DNA Complex Obtained by Small-Angle Neutron or X-Ray Scattering

Tatsuhito Matsuo et al. Methods Mol Biol. 2022.

Abstract

Small-angle scattering is a powerful technique to obtain structural information on biomacromolecules in aqueous solution at the sub-nanometer and nanometer length scales. It provides the sizes and overall shapes of the scattering particles. While small-angle X-ray scattering (SAXS) has often been used for structural analysis of a single-component system, small-angle neutron scattering (SANS) has been used to reveal the internal organization of a multicomponent system such as protein-protein and protein-DNA complexes. This is due to the fact that the neutron scattering length is largely different between hydrogen and deuterium, and thus it allows to make a specific component in complexes "invisible" to neutrons by changing the H2O/D2O ratio in the solvent with or without molecular deuteration. In this chapter, we describe a method to characterize the biomolecular structures using SANS and SAXS, in particular, focusing on fibrillar proteins such as bacterial amyloids and their complexes with nucleic acids.

Keywords: Bacterial amyloid; Protein-DNA complex; Small-angle X-ray scattering; Small-angle neutron scattering; Structural analysis.

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