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. 1987 Apr;6(4):835-40.
doi: 10.1002/j.1460-2075.1987.tb04828.x.

DNA-mediated transformation of the basidiomycete Coprinus cinereus

D M Binninger, C Skrzynia, P J Pukkila, L A Casselton

DNA-mediated transformation of the basidiomycete Coprinus cinereus

D M Binninger et al. EMBO J. 1987 Apr.

Abstract

We have developed a simple and efficient transformation system for the agaric fungus, Coprinus cinereus. Protoplasts were prepared from asexual spores that harbor one or two mutations in the structural gene for tryptophan synthetase. The protoplasts can be stably transformed using the cloned Coprinus gene at a frequency of 1 in 10(4) viable protoplasts. A variety of molecular events accompanies the formation of stable transformants, including insertion of the transforming DNA at the homologous locus. The transforming DNA is stable through cell division, mating, fruiting body formation, and meiosis.

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References

    1. J Mol Biol. 1977 Jun 15;113(1):237-51 - PubMed
    1. Proc Natl Acad Sci U S A. 1954 Mar;40(3):192-200 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5259-63 - PubMed
    1. Nucleic Acids Res. 1981 Jul 10;9(13):2989-98 - PubMed
    1. J Mol Biol. 1981 Feb 5;145(4):619-32 - PubMed

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