The Screening of Therapeutic Peptides for Anti-Inflammation through Phage Display Technology

Abstract

For the treatment of inflammatory illnesses such as rheumatoid arthritis and carditis, as well as cancer, several anti-inflammatory medications have been created over the years to lower the concentrations of inflammatory mediators in the body. Peptides are a class of medication with the advantages of weak immunogenicity and strong activity, and the phage display technique is an effective method for screening various therapeutic peptides, with a high affinity and selectivity, including anti-inflammation peptides. It enables the selection of high-affinity target-binding peptides from a complex pool of billions of peptides displayed on phages in a combinatorial library. In this review, we will discuss the regular process of using phage display technology to screen therapeutic peptides, and the peptides screened for anti-inflammation properties in recent years according to the target. We will describe how these peptides were screened and how they worked in vitro and in vivo. We will also discuss the current challenges and future outlook of using phage display to obtain anti-inflammatory therapeutic peptides.

Keywords: anti-inflammatory peptides; inflammation pathway; inflammation target; phage display; therapeutic peptide.

Figure 1

This figure depicts the two most commonly used methods for screening anti-inflammatory therapeutic peptides. The left is the flowchart of screening the peptide from the random peptide library; the right is the flowchart of screening the peptide from the construct library.